不同离体时间和抗凝剂浓度对血小板激活的影响

Effect of in vitro time and anticoagulant concentration on platelet activation

摘要:

目的：探索不同离体时间和不同抗凝剂浓度对血小板激活的影响。方法：利用流式细胞术和单克隆抗体检测85例健康成人的外周血血小板，在不同离体时间和不同枸橼酸钠浓度的作用下，血小板表面糖蛋白PAC-1、CD62p、CD63以及凝血酶敏感蛋白（Thrombin-sensitive protein，TSP）的表达量。结果：相同离体时间下，血小板的激活程度随枸橼酸钠浓度（0.109～0.872 mol/L）的增加而增加，在大于0.872 mol/L浓度枸橼酸钠浓度下，血小板的激活状态变化不明显。离体2 h内血小板表现为轻微增加的激活，2～8 h内血小板的激活上升明显，8 h后其状态基本维持不变。结论：一定范围内增加抗凝剂浓度和延长离体时间会促进血小板的激活，要控制最合适的抗凝剂浓度和最短的离体时间来检测血小板相关指标，并选择适当的储存方法保存血小板以降低其因为离体而发生的活化。

Abstract:

Objective：To investigate the effect of in vitro time and anticoagulant concentration on platelet activation. Methods：The pe－ripheral blood platelet of 85 healthy adults was exposed to different in vitro time and different concentrations of sodium citrate，then flow cytometry（FCM） and monoclonal antibodies（McAb） were used to detect the expression levels of platelet surface glycoprotein protein PAC-1，CD62p，CD63，thrombin-sensitive protein（TSP）. Results：Under the same in vitro time，platelet activation was in－creased with the concentration of sodium citrate increasing from 0.109 mol/L to 0.872 mol/L. Platelet activation did not change signif－icantly when the citrate concentration was higher than 0.872 mol/L. Platelet activation was slight increased within 2 h in vitro，signif－icantly increased within 2-8 h，remained unchanged after 8 h. Conclusions：Increasing the anticoagulant concentration within certain range and extending time in vitro can enhance platelet activation. We should choose the most appropriate anticoagulant concentration and the shortest time in vitro to test the platelet-related indicators and select appropriate storage method to save the platelet in order to reduce its activation.