ELITE PRO血凝仪2种测定纤维蛋白原方法结果的比较

Comparsion between two fibrinogen detection methods with ELITE PRO coagulometer

摘要:

目的：研究贝克曼库尔特ACL ELITE PRO全自动血凝仪，简称ELITE PRO。用 凝血酶原时间（Prothrombin time，PT）衍算法（PT-Fib）测定纤维蛋白原（Fibrinogen，Fib）含量的精密度及其与凝血酶法（Clauss法）检测结果的相关性。方法：用 Clauss法及 PT-Fib法同时测定232例标本的Fib含量，对其结果的相关性进行分析，得出 PT-Fib法测定 Fib在ELITE PRO上的可信范围。结果： PT-Fib法检测结果精密度高，当 Fib 含量在1.5～4.5 g/L时，PT-Fib法与 Clauss法的结果接近,两者测定结果正相关；当 Fib＜1.5 g/L或＞4.5 g/L时，PT-Fib法的结果高于 Clauss法。结论：当 PT-Fib法测定的 Fib含量在1.5～4.5 g/L时结果可靠，可直接用于临床报告；当 PT-Fib法测定的 Fib结果＜1.5 g/L或＞4.5 g/L时结果不稳定，应用 Clauss法进一步复查。2种方法结合使用既可保证 Fib检测速度的迅速、结果的准确，也可达到节约试剂的目的。

Abstract:

Objective：To evaluate the accuracy of using PT-Fib method to determine fibrinogen（Fib） content in plasma samples with ACL ELITE PRO automatic coagulometer（ELITE PRO） and its correlation with results of Clauss method. Methods：Clauss method and PT-Fib method were used to determine Fib content in 232 plasma fibrinogen samples. Correlation analysis was made to get the confidential range in ELITE PRO when detecting Fib content by PT-Fib method. Results：The coefficient of variation of PT-Fib method was 3.8% in ELITE PRO. When the content of Fib was 1.5-4.5 g/L，there was no significant difference between Clauss method and PT-Fib method and results of the two methods were positively coorelated；when the content of Fib was<1.5 g/L or>4.5 g/L，PT-Fib method results was significant higher than that of Clauss method（P >0.05）. Conclusions：When Fib content is 1.5-4.5 g/L detected by PT-Fib method，the result is reliable and can be directly used in clinical report；when Fib content is<1.5 g/L or＞r 4.5 g/L detected by PT-Fib method，the result is unreliable and shoul be rechecked by Clauss method. Combined use of the two methods can be sim－pler，quicker and more accurate and economical in detecting Fib.