YAP基因及蛋白在肾癌中的表达及意义

Expression and clinical significance of gene and protein in human renal carcinoma tissues

摘要:

目的：观察Ｙes相关蛋白（Yes-associated protein，YAP）基因及蛋白在肾癌组织中的表达情况及其意义。方法：采用RT-PCR技术及免疫组化检测42例肾癌组织和癌旁正常肾组织中YAP基因及蛋白的表达情况。结果：YAP基因在肾癌组织中阳性表达率为88.10%，而在癌旁正常组织中仅为14.29%。YAP蛋白在肾癌组织中阳性率也高于癌旁正常组织（95.23% vs.30.95%），两者相比有统计学差异（P<0.05）。结论：YAP可能与肾癌的发生发展密切相关，YAP在肾癌中的异常表达可为肾癌的诊断，治疗提供实验依据，尤其为诱导细胞凋亡和基因治疗肾癌打下基础。

Abstract:

Objective：To observe the expression and clinical significance of Yes-associated protein（YAP） gene and protein in human renal carcinoma tissues. Methods：The expressions of YAP mRNA were measured by reverse transcription-polymerase chain reac－tion （RT-PCR） and the expressions of YAP protein were detected by immunohistochemistry in 42 cases of human renal carcinoma tis－sues and their corresponding adjacent non-malignant tissues. Results：The positive expression rates of YAP mRNA in renal carcino－ma tissues and adjacent non-malignant tissues were 88.10% and 14.29% respectively. The positive expression rate of YAP protein was higher in renal carcinoma tissues than in adjacent non-malignant tissues（95.23% vs. 30.95%），with statistical differences（P<0.05）. Conclusions：The expression of YAP might be associated with the generation and development of renal carcinoma and its abnormal expression can provide references for the diagnosis and treatment of renal carcinoma. Especially，it can establish the theoretical basis for inducement of cell apoptosis and gene therapy of renal carcinoma.