慢性间歇性缺氧对大鼠食管及其白介素-6、超敏C-反应蛋白表达的影响

Effect of chronic intermittent hypoxia on esophagus and expressions of IL-6，hs-CRP in rats

摘要:

目的：制作可用于常压慢性间歇性缺氧（Chronic intermittent hypoxia，CIH）研究的动物模型。探讨CIH与胃食管反流病（Gastroesophageal reflux disease，GERD）的关系及CIH对食管白介素-6（Interleukin-6，IL-6）、超敏C-反应蛋白（High sensitiv－ity-C reactive protein，hs-CRP）表达的影响。方法：将24只成年雄性SD大鼠随机分成3组，即空白对照（Unhandled control，UC）组（正常饲养4周）、CIH组、建立CIH模型（饲养4周）和复氧（Removal of hypoxia group，RH）组（CIH 4周后正常饲养4周），所有大鼠均在实验结束后测定各组食管下段pH值，观察食管病理变化，检测食管组织IL-6及hs-CRP的表达水平。结果：CIH组食管组织发生明显炎症改变，UC组和RH组炎症不明显。pH值明显下降（P<0.05），IL-6及hs-CRP的表达明显增高（P<0.01），RH组与UC组比较均无明显差异（P >0.05）。结论：CIH可致食管发生炎性改变，IL-6及hs-CRP参与了CIH胃食管反流的发生。

Abstract:

Objective：To set up the rat model of chronic intermittent hypoxia（CIH） under normal pressure，to study the relationship between CIH and gastroesophageal reflux disease（GERD） and to explore the effect of CIH on expression of interleukin-6（IL-6） and high sensitivity-C reactive protein（hs-CRP） in rat. Methods：Twenty-four sex maturation rats were randomly divided into unhandled control group（UC group，normal feeding for 4 weeks），CIH group（CIH for 4 weeks），removal of hypoxia group（RH group，normal feeding for 4 weeks after CIH for 4 weeks）. The pH values and the expressions of IL-6 and hs-CRP in the inferior esophageal segment were determined and observed. Results：The inflammation was more obvious in CIH group than in UC group and RH group. The pH values were significantly lowered（P<0.05） and the expressions of IL-6 and hs-CRP were significantly elevated（P<0.01），with no dif－ference between UC group and RH group（P >0.05）. Conclusions：CIH may induce the inflammation change of esophagus and IL-6 and hs-CRP participate in GERD’s development.