三七总皂苷对肥胖小鼠代谢紊乱及脂肪组织炎性因子表达的改善作用

Effects of panax notoginseng saponins on diet induced metabolic dysfunction and expression of inflammatory cytokines in adipose tissue of obese mice

摘要:

目的：观察三七总皂苷（Panax notoginseng saponins，PnS）对肥胖小鼠代谢紊乱和脂肪组织炎性细胞因子表达的影响，探讨PnS改善肥胖的作用机制。方法：将C57BL/6小鼠分别用标准饮食（Standard diet，SD）和高脂饮食（High fat diet， HFD）喂养10周，HFD组每天处以低和高剂量PnS（100、200 mg/kg），SD组每天处以200 mg/kg的PnS，对照组处以等体积的生理盐水。观察进食量、体脂、给药前后体重的变化，全自动生化仪检测血清脂质含量，采用ELISA法测定血清胰岛素和炎症因子肿瘤坏死因子-?琢（Tumor necrosis factor，TNF-?琢）的水平，实时荧光定量PCR检测巨噬细胞趋化蛋白-1（Monocyte chemoattractant protein-1，MCP-1）及TNF?琢 mRNA的表达。结果：低和高剂量PnS处理２周能降低HFD小鼠的体脂量，降低血清胰岛素和TNF－?琢的水平，使脂肪组织MCP-1和TNF-?琢 mRNA表达明显降低，血脂成分有不同程度的改善且高剂量组优于低剂量组。结论：PnS对高脂饮食诱发的代谢紊乱有一定程度的改善作用，高剂量组的改善作用优于低剂量。

Abstract:

Objective：To investigate the effects of panax notoginseng saponins（PnS） on diet induced metabolic dysfunction and ex－pression of inflammatory cytokines in adipose tissue of obese mice and to discuss the function of PnS in improving obesity. Methods：C57BL/6 mice were randomized into standard diet（SD） and high fat diet（HFD）. Ten weeks afterwards，HFD mice were treated with vehicle（HFD-Veh），low doses（100 mg/kg，HFD-PnS 100） and high doses（200 mg/kg，HFD-PnS 200） of PnS while SD mice were treated with vehicle（SD-Veh） and high doses of PnS（SD-PnS 200）. The mice in control group were treated with the same volume of saline. Body weight and fat mass in each group were detected. Serum lipid and glucose levels were detected using automatic bio－chemistry analyzer. Serum insulin and tumor necrosis factor-?琢（TNF-?琢） levels were assayed by enzyme-linked immunosorbent assay（ELISA）. The expression of monocyte chemoattractant protein－1（MCP-1） and TNF-?琢 mRNA in epididymis adipose tissue were de－tected by real-time quantitative PCR. Results：Two weeks after treatment with PnS，the body weight，fat mass，serum insulin and TNF-?琢 level as well as the expression of MCP-1 and TNF-?琢 mRNA of obese mice in both high and low dose group were significantly de－creased. The contents of serum lipid and glucose were improved and the effect was better in high dose group than in low dose group. Conclusions：PnS has beneficial effects on diet induced metabolic dysfunction and high doses of PnS are superior to low doses.