吗啡成瘾及癫痫造模对ICR小鼠学习记忆能力的相关性研究

Correlation between Morphine addiction，epilepsy model and learning and memorizing of ICR mice

摘要:

目的：观察吗啡成瘾及癫痫对ICR小鼠的空间学习记忆能力的影响是否具有相关性。方法： 将40只小鼠随机分成吗啡成瘾继发癫痫组、吗啡成瘾组、癫痫组和空白对照组，每组10只。用连续注射吗啡建立吗啡成瘾模型，用匹罗卡品（Pilo－carpine，PLO）建立癫痫模型。用水迷宫实验检测各组小鼠的记忆能力。结果：吗啡成瘾继发癫痫小鼠与癫痫小鼠水迷宫逃避潜伏期时间相对比明显延长（P<0.05）。结论：吗啡成瘾与癫痫对ICR小鼠的学习记忆能力损害具有协同作用。

Abstract:

Objective：To create Morphine addiction model by conditioned place preference firstly and then to create epilepsy model on the basis of Morphine addiction，finally to study whether Morphine addiction and epilepsy exert effects on spatial learning and memorizing of institute of cancer research（ICR） mice from behavioral by water maze. Methods：Totally 40 ICR mice having excellent performance by early platform of natural preference and training of water maze were selected. All these mice were equally divided into Morphine addiction complicated with epilepsy group, Morphine addiction group, epilepsy group and control group（n=10）. Seven days continuous injection of Morphine was used to create Morphine addiction model while Pilocarpine was injected to create epilepsy model. Water maze test was performed to observe the learning and memorizing of mice. Results：Water maze escape latency was longer in Morphine addiction complicated epilepsy mice than in epilepsy mice. Conclusion：Morphine addition and epilepsy exert synergistic effect on learning and memory of ICR mice.