新疆地区人乳头瘤病毒感染的基因型研究

Genotype of human papilloma virus infection in Xinjiang

摘要:

目的：应用PCR-反向点杂交（PCR-reverse dot blot，PCR-RDB）技术对新疆地区人乳头瘤病毒（Human papilloma virus－es，HPV）进行基因分型。方法：收集疑似HPV感染病例标本335例，提取HPV基因组DNA，应用PCR-RDB技术扩增HPV的L1基因并进行分型，计算不同基因型HPV的感染率，组间率的比较采用χ2检验。结果：335例病例标本中检出HPV阳性标本108例，阳性率为32.2%，共检出HPV16、18、33、45、56、68、6、11和66共9种基因型，其中HPV16、18和56亚型的感染较高。分组统计结果显示，HPV阳性率和高危型HPV的检出率在不同类型疾病患者中是不同的，在尖锐湿疣、高度子宫颈上皮内瘤变Ⅱ/Ⅲ和宫颈癌患者中HPV阳性率和高危型HPV的检出率均显著高于宫颈炎组和宫颈糜烂疾病组（P<0.05），而随着年龄的不断增加，HPV感染率降低。HPV的感染率在19～36年龄组较高，随着年龄的增加，HPV感染率有降低趋势。结论：在本地区宫颈病变患者中HPV的感染率较高，而基于PCR-RDB的基因分型可为HPV感染的诊断、治疗和随访提供依据。

Abstract:

Objective：To investigate the genotypes of human papilloma viruses（HPV）in women with different gynecologic diseases in Xinjiang by PCR-reverse dot blot（PCR-RDB）. Methods：Clinical specimens from 335 women with suspected symptoms of gynecologic disorders were collected and HPV DNA（L1） was isolated and subjected to genotyping by PCR-RDB. HPV infection rates were cal－culated and statistical analysis of χ2 test was performed to compare the HPV infection rates among different groups. Results：Totally 108 specimens were identified to be HPV positive and the posotive rate was 32.2%（108/335）. Nine kinds of HPV genotypes（HPV 16，18，33，45，56，68，6，11，66） were identified by PCR-RDB and HPV 16，18，56 had higher infection rates than other HPV genotypes. Statistic analysis showed that patients with condyloma，cervical intraepithelial neoplasiaⅡ/Ⅲ and cervical cancer had significantly higher HPV positive rates and high-risk HPV infection rates than those with cervicitis or cervical erosion（P＜0.05）. Additionally，HPV infection rates were decreased with the increasing of age. Conclusions：HPV infection is common in patients with different uter－ine-cervical diseases in Xinjiang and HPV genotyping by PCR-RDB could provide epidemiological evidence for the diagnosis，treat－ment and follow-up of HPV infection.