小鼠脑组织细胞色素Ｃ氧化酶活性、线粒体细胞色素含量与年龄关系

Relationship among CCO activity，contents of Cyt C in mitochondria of brain tissues and mice age

摘要:

目的：研究小鼠脑细胞色素C氧化酶（Cytochrome C oxidase，CCO）活性、线粒体细胞色素C（Cytochrome C，Cyt C）含量与年龄的关系，为法医学年龄推断提供新的思路。方法：利用紫外分光光度法检测了1、3、6、9、12月龄昆明小鼠的脑组织CCO活性及线粒体Cyt C含量。结果：小鼠脑组织CCO活性、线粒体Cyt C含量，随着年龄增大而逐渐降低。结论：脑组织CCO活性及线粒体Cyt C含量有望用于年龄推断。

Abstract:

Objective：To provide new ideas for forensic age estimation by studying relationship among cytochrome C oxidase（CCO）activity，contents of cytochrome C（Cyt C） in mitochondria of brain tissues and mice age. Methods：CCO activity and contents of Cyt C in mitochondria of brain tissues of 1，3，6，9，12 months old Kunming mice were detected using UV spectrophotometry. Results：CCO activity and contents of Cyt C in mitochondria of brain tissues were decreased gradually with the increase of mice age. Conclusion：CCO activity and contents of Cyt C in mitochondria of brain tissues may be used for age estimation.