生物钟Per1基因在人颊部鳞状细胞癌中的表达及临床意义

Expressions and clinical significances of circadian clock gene Per1 in patients with oral squamous cell carcinoma

摘要:

目的：探讨生物钟基因Per1在口腔颊鳞癌组织中的表达及意义。方法：应用免疫组化SP法和Western blot法检测38例口腔颊鳞癌及其癌旁正常颊黏膜组织中Per1的表达情况，并结合临床病理指标进行探讨。结果：Per1在颊鳞癌中的表达显著低于在癌旁正常颊黏膜组织中的表达（P=0.025）。Per1在Ⅰ、Ⅱ期颊鳞癌中的表达显著高于在Ⅲ、Ⅳ期的表达（P=0.007），在T1、T2期颊鳞癌组织中的表达显著高于在T3、T4期中的表达（P=0.030），在无淋巴结转移者的表达显著高于在有淋巴结转移者中的表达（P=0.008），Per1的表达与患者性别、年龄及病理分级无明显相关性（P均大于0.05）。结论：Per1的表达在口腔鳞癌的发生发展，浸润、转移中可能占重要作用，为口腔颊鳞癌的诊断、预后评估和治疗提供了新的思路。

Abstract:

Objective：To investigate the expressions and significances of circadian clock gene Per1 in patients with oral squamous cell carcinoma（OSCC）. Methods：Expressions of Per1 in 38 cases of buccal squamous cell carcinoma and the adjacent non-tumor tissues were examed by immunohistochemical SP method and Western blot method and the significances of Per1 were studied combined with the clinicopathological parameters. Results：Expressions of Per1 were significantly decreased in buccal squamous cell carcinoma tis－sues than in adjacent non-tumor tissues（P=0.025）. In buccal squamous cell carcinoma，expressions of Per1 were significantly higher in stageⅠ，Ⅱ period and T1，T2 grade than in stage Ⅲ，Ⅳ period and T3，T4 grade（P=0.007）（P=0.030），meanwhile the expressions of Per1 were significantly higher in group without lymph node metastasis than in group with lymph node metastasis（P=0.008）. Ex－pression of Per1 was in no correlation with patients’ gender，age and pathological grade（P >0.05）. Conclusions：Expression of Per1 may play an important role in the development，invasion and metastasis of patients with OSCC，and may provide a new way of thinking for OSCC diagnosis，prognosis assessment and treatment.