淫羊藿总黄酮对多囊卵巢综合征大鼠性激素水平的影响

Effects of epimedium total flavonoids on sex hormone in rats with polycystic ovary syndrome

摘要:

目的：探讨淫羊藿总黄酮对多囊卵巢综合征（polycystic ovary syndrome，PCOS）大鼠性激素水平的影响。方法：55只SD大鼠连续灌胃1 mg/kg来曲唑21 d，建立PCOS大鼠模型；选取造模成功的50只大鼠随机分为PCOS模型组，达英-35组，淫羊藿总黄酮低、中、高剂量组（50、100、200 mg/kg），另设正常对照组（n=10）。连续治疗21 d后，测量大鼠体质量、卵巢重量，计算卵巢指数；采用放射免疫法测定各组大鼠血清睾酮（testosterone，T）、促卵泡生成素（follicle stimulating hormone，FSH）、雌二醇（estradiol，E2）、黄体生成素（luteinizing hormone，LH） 水平。结果：与正常对照组相比，PCOS组大鼠卵巢重量和卵巢指数均显著升高（P<0.05），血清T、LH水平显著升高（P<0.001），E2水平明显下降（P<0.01），LH/FSH比值明显高于正常组（P<0.01）；经21 d治疗后，与PCOS模型组相比，200 mg/kg淫羊藿总黄酮组大鼠卵巢重量和卵巢指数均显著下降（P<0.05），血清T、LH水平显著降低（P<0.01），E2显著升高（P<0.01），LH/FSH比值亦明显下降（P<0.01）。100 mg/kg和50 mg/kg淫羊藿总黄酮作用较弱。结论：淫羊藿总黄酮可改善PCOS大鼠高雄激素血症，调节LH/FSH异常，升高E2，改善卵巢功能。

Abstract:

Objective：To explore the effects of epimedium total flavonoids on sex hormone in rats with polycystic ovary syndrome （PCOS）. Methods：Letrozole（1 mg/kg） was intragastrically administered in SD rats for 21 d to establish PCOS model. The PCOS rats were randomly divided into 5 groups（n=10）：PCOS model group，Diane-35 group（240 mg/kg），epimedium flavonoids group（50，100，200 mg/kg）. In addition，normal control group（n=10） was established. After continuous oral administration for 21 d，rat body weight and ovarian weight were measured and ovarian index was calculated. Serum testosterone（T），follicle stimulating hormone（FSH），estra－diol（E2），and luteinizing hormone（LH） were detected with radioimmunoassay. Results：Ovarian weight and ovarian indexes were sig－nificantly increased in PCOS group compared with those in normal control group（P<0.05）. Moreover，PCOS group had higher serum T，LH（P<0.001），LH/FSH（P<0.01） and lower E2 level（P<0.01）. After administration for 21 d，ovarian weight，ovarian index（P<0.05），serum T，LH（P<0.01） and LH/FSH（P<0.01） were decreased in 200 mg/kg epimedium flavonoids group compared with those in PCOS model group，but E2 level was significantly increased（P<0.01）. However，100 mg/kg and 50 mg/kg epimedium flavonoids had weak effects. Conclusions：Epimedium flavonoids can improve hyperandrogenism in PCOS rats，regulate LH/FSH，elevate E2 and improve ovarian function.