HIF-1a、MMP-9、MVD在肾细胞癌中的表达及临床意义

Expressions of HIF-1a，MMP-9 and MVD in renal carcinoma and their clinical significances

摘要:

目的：探讨缺氧诱导因子-1a（hypoxia inducible factor-1a，HIF-1a）和基质金属蛋白酶-9（matrix metalloproteinases-9，MMP-9）在肾癌中的表达及其与微血管密度（microvessel density，MVD）和肿瘤进展的关系。方法：应用免疫组织化学法检测60例肾癌组织和20例癌旁正常肾组织中HIF-1a和MMP-9的表达及其血管生成情况，并分析三者与各临床病理参数之间的关系。结果：HIF-1a在肾癌组织和癌旁正常组织中的表达率分别为58.33%，0%；MMP-9在肾癌组织和癌旁正常组织中的表达率分别为63.33%，20%；肾癌组织和癌旁正常组织中MVD均值分别为（37.93±11.94）条/HP，（22.30±7.89）条/HP，HIF-1a、MMP-9及MVD在肾癌中的表达与癌旁正常组织中的表达分别比较，差异均有统计学意义（P<0.05）；并且与病理分期和淋巴结转移具有相关性。结论：HIF-1a、MMP-9和MVD在肾癌组织中呈持续高表达，HIF-1a和MMP-9参与肾癌新生血管的形成，并与侵袭转移关系密切，三者联合检测是判断肾癌侵袭转移有价值的指标。

Abstract:

Objective：To study the expressions of hypoxia inducible factor-1a（HIF-1a） and matrix metalloproteinases-9（MMP-9） in renal carcinoma tissues and to study their relationship with microvessel density（MVD） and tumor progression. Methods：Immunohis－tochemical staining was used to detect the expressions of HIF-1a and MMP-9 as well as angiogenesis in 60 cases of renal carcinoma tissues and 10 cases of adjacent normal kidney tissues and the relationships between HIF-1a，MMP-9，MVD and clinicopathological parameters were analyzed. Results：Positive expression rates of HIF-1a in renal carcinoma tissues and adjacent cancer tissues were 58.33% and 0% respectively；positive expression rates of MMP-9 in renal carcinoma tissues and adjacent cancer tissues were 63.33% and 20% respectively；mean values of MVD in renal carcinoma tissues and adjacent cancer tissues were （37.93±11.94）strip/HP and （22.30±7.89）strip/HP respectively. There were statistical significances in expressions of HIF-1a，MMP-9，MVD between renal carcinoma tissues and adjacent cancer tissues（P<0.05） and they were correlated with pathology classification and lymph node metastasis. Conclusions：Increased expressions of HIF-1a，MMP-9，MVD are found in renal carcinoma tissues. HIF-1a and MMP-9 are involved in the angiogenesis of renal carcinoma and are correlated with cancer invasion and metastasis. Combined detection of HIF-1a，MMP-9 and MVD can provide valuable index for judging invasion and metastasis of renal carcinoma.