精氨酸甲基转移酶4及其产物不对称二甲基精氨酸在多囊卵巢综合征中的初步探讨

Protein aiginine methyltransferase 4 and methylated metabolite asymmetric dimethylarginines in polycystic ovary syndrome

摘要:

目的：研究多囊卵巢综合征（polycystic ovary syndrome，PCOS）患者、人正常对照组血清及PCOS模型大鼠、大鼠对照组血清中不对称二甲基精氨酸（asymmetric dimethylarginines，ADMA）的含量，以及PCOS模型大鼠卵巢组织中蛋白质精氨酸甲基转移酶4（protein aiginine methyltransferase 4，PRMT4）的表达情况，研究二者与PCOS之间的关系。方法：运用ELISA测定49例PCOS患者、20例人正常对照组以及20例PCOS模型大鼠、20例大鼠对照组血清中ADMA的含量，并采用免疫组化SP法测定PCOS模型大鼠卵巢组织中PRMT4的表达。结果：（1）人血清中ADMA含量PCOS组为（0.49±0.35） umol/L，对照组为（0.26±0.09） umol/L，两组差异具有统计学意义（P=0.00）；（2）大鼠血清中，PCOS模型组ADMA含量为（0.55±0.14） umol/L，对照组为（0.23±0.08） umol/L，两组差异具有统计学意义（P=0.00）；（3）大鼠卵巢组织中，模型组PRMT4积分光密度（integral optical den－sity，ＩＯＤ）值为（44.96±9.40） umol/L，对照组为（18.27±3.83）umol/L，两组差异具有统计学意义（P=0.00）。结论：PRMT4的含量累积可能导致其产物ADMA的生成增加，共同参与PCOS血管内皮功能紊乱产生，从而促进PCOS的发展。

Abstract:

Objective：To investigate levels of asymmetric dimethylarginines（ADMA） in serum of women with and without polycystic ovary syndrome（PCOS），PCOS model rats and control rats，to explore expressions of protein aiginine methyltransferase 4（PRMT4） in ovarian tissues of PCOS model rats and to explore relationship between levels of ADMA and PRMT4 and PCOS. Methods：ELISA was used to investigate levels of ADMA in serum of 49 PCOS patients，20 normal patients，20 PCOS model rats and 20 normal rats. Ex－pressions of PRMT4 in ovarian tissues were detected by SP immunohistochemical method（including 20 PCOS model rats and 20 con－trol rats）. Results：（１）In human serum，ADMA concentrations were （0.49±0.35）umol/L and （0.26±0.09） umol/L in PCOS group and control group respectively，with significant differences between two groups（P=0.00）. （２）In rat serum，ADMA concentrations were （0.55±0.14） umol/L and （0.23±0.08） umol/L in PCOS group and control group respectively，with significant differences between two groups（P=0.00）. （３）In rat ovarian tissues，PRMT4 integral optical density values were （44.96±9.40） umol/L and （18.27±3.83） umol/L in PCOS group and control group respectively，with significant differences between two groups（P=0.00）. Conclusions：In ovar－ian tissues，increased expressions of PRMT4 may bring about elevated ADMA level in serum，which causes endothelial dysfunction of PCOS and affects PCOS development.