高温处置后人体组织DNA提取研究

Research on extracting DNA of human tissues after high-temperature disposal

摘要:

目的：研究高温后人体组织DNA的提取方法对DNA-STR分型的影响。方法：用不同浓度TritonX-100处理高温后的人体组织样本，采用Chelex-100提取DNA的方法，经DNA-STR复合扩增，用ABI-3130序列分析仪电泳分离并运用Gene mapper ID V3.2基因分析软件对基因进行分型。结果：用1% TritonX-100+Chelex-100处理样本后提取DNA，再经QIA quick PCR纯化后可检出全部基因座且基因座能较平衡地扩增。结论：该方法简便易行，实验效果好，是法科学领域中一种值得推荐的方法，尤其适合检案。

Abstract:

Objective：To study the effects of DNA extraction methods on deoxyribonucleic acid-short tandem repeats（DNA-STR） typ－ing of human tissue after high-temperature disposal. Methods：Human tissue samples after high-temperature disposal were initially handled with different concentrations of TritonX-100. DNA was extracted using the method of Chelex-100 and was amplified by re－combination of DNA-STR. Then，ABI-3130 sequence analyzer was used for electrophoresis separation and Gene mapper ID V3.2 gene analysis software was used for gene typing. Results：1% TritonＸ-100+Chelex-100 was used to process the samples before DNA ex－traction. All loci could be detected after QIA quick PCR purification and the loci could be balancedly amplified. Conclusions：This is a simple method with satisfied results，which is recommended in the field of forensic science，especially for the prosecution case.