新城疫病毒抑制肝星状细胞活化的初步研究

Inhibition of activation of hepatic stellate cell by newcastle disease virus

摘要:

目的：探讨新城疫病毒（newcastle disease virus，NDV）在活化肝星状细胞（hepatic stellate cell，HSC）中的复制对HSC的活化增殖以及细胞生物学功能的影响。方法：用不同滴度的NDV溶瘤株Italien（NDV-Italien）感染经转化生长因子-β1（trans－formation growth factor-β1，TGF-β1）刺激活化的人HSC系LX-2。MTT法检测NDV 对细胞增殖率的影响。免疫荧光显微镜技术检测活化标志物α-SMA的表达变化。结果：NDV的感染抑制了LX-2细胞的增殖，细胞增殖率和病毒滴度呈负相关。与未刺激组相比，NDV在TGF-β1刺激的LX-2细胞中的增殖抑制率提高，LX-2细胞的活化标志物α-SMA的表达显著性下调。结论：NDV在活化LX-2细胞中的复制具有抑制细胞增殖和HSC活化的作用。

Abstract:

Objective：To explore the influence of newcastle disease virus（NDV） replication in LX-2 cells on the viability and biologi－cal function of hepatic stellate cell（HSC）. Methods：Transforming growth factor-β1（TGF-β1）-stimulated LX-2 cells were infected by different titres of oncolytic NDV-Italien，the proliferation rate of LX-2 cells was detected by MTT assay. Immunofluorescent tech－nique was used to detect the expression of activation markers α-SMA in LX-2 cells. Results：NDV infection in LX-2 cells inhibited the cell proliferation in a dose-dependent manner. The growth inhibition rate of activated LX-2 cells was increased，the expression of activation markers α-SMA of LX-2 cells was down-regulated dramaticlly compared with those of controls. Conclusions：Replication of NDV in activated LX-2 cells inhibits the cell proliferation and attenuates the activation of LX-2 cells.