可溶性细胞间黏附分子-1在乙肝相关性肝病患者发病中作用的初步研究

Preliminary study on the role of serum soluble intercellular adhesion molecule-1 in the pathogenesis of hepatitis B virus related liver diseases

摘要:

目的：通过检测乙肝相关性肝病患者血清可溶性细胞间黏附分子-1（soluble intercellular adhesion molecule-1，sICAM-1）的水平，探讨sICAM-1在乙肝相关性肝病患者发病中的作用。方法：选择慢性乙型肝炎、乙型肝炎肝硬化及肝衰竭患者各30例，另选择健康献血员30例作对照组，应用酶联免疫吸附法（enzyme-linked immunosorbent assay，ELISA）法检测血清sICAM-1的水平。结果：慢性乙型肝炎、乙型肝炎肝硬化、肝衰竭患者血清sICAM-1水平较正常对照组均明显升高（P=0.029、P=0.000 07、P=0.000 1），并与丙氨酸氨基转移酶（alanine aminotransferase，ALT）、天门冬氨酸氨基转移酶（aspartate amino transferase，AST）、总胆红素（total bilirubin，TBIL）呈正相关（ALT：rs=0.240，P=0.023；AST：rs=0.225，P=0.033；TBIL：rs=0.262，P=0.013），与白蛋白（al－bumin，ALB）、凝血酶原活动度（prothrombin activity，PTA）呈负相关（ALB：rs=-0.297，P=0.005；PTA：rs=-0.218，P=0.039）。结论：sICAM-1可能参与了乙型肝炎免疫损伤过程，并可能在肝纤维化、肝硬化形成过程中发挥重要作用。检测乙肝相关性肝病患者血清sICAM-1水平对判断患者病情有一定临床意义。

Abstract:

Objective：To explore the role of serum intercellular adhesion molecule-1（sICAM-1） in the pathogenesis of hepatitis B virus related liver diseases by detecting the levels of sICAM-1 in patients with hepatitis B virus related liver diseases. Methods：Lev－els of sICAM-1 were measured by enzyme-linked immunosorbent assay（ELISA） in the 120 subjects（30 patients with chronic hep－atitis B，30 patients with liver cirrhosis，30 patients with liver failure and 30 health controls）. Results：Serum levels of sICAM-1 were higher in patients with chronic hepatitis B，cirrhosis and liver failure than in healthy controls（P=0.029，P=0.000 07，P=0.000 1）. In patients with hepatitis B virus related liver diseases，sICAM-1 levels were positively correlated with levels of alanine aminotransferase（ALT），aspartate aminotransferase（AST），total bilirubin（TBIL）（ALT：rs=0.240，P=0.023；AST:rs=0.225，P=0.033；TBIL：rs=0.262，P=0.013）and were negatively correlated with the levels of albumin（ALB），prothrombin activity（PTA）（ALB：rs=-0.297，P=0.005；PTA：rs=-0.218，P=0.039）. Conclusions：sICAM-1 may interfere the procedure of immunological damage of hepatitis B and plays an impor－tant role in the pathogenesis of live fibrosis and cirrhosis. Examination of serum levels of sICAM-1 in patients with hepatitis B virus related liver diseases can be used to judge the patients’ condition.