Objective：To study the clinical value of serum markers（CA15-3，CA125，CYFRA21-1，CA72-4 and tumor associated ma－terial（TAM）） in the diagnosis of breast cancer at Ⅲ+Ⅳ stages and during postoperative follow-up. Methods：Serum levels of CA15-3，CA125，CYFRA21-1，CA72-4（with electrochemiluminescence immunoassay） and TAM （with biochemical process） were detected in 104 patients with breast cancer（58 patients at stagesⅠ+Ⅱ，46 patients at stages Ⅲ+Ⅳ），60 patients with benign breast tumor and 65 patients with follow-up recurrence. Eighty patients received chemotherapy including those with breast cancer at stages Ⅲ+Ⅳand those with postoperative recurrence（33 patients in invalid group and 47 patients in effective group）. Results：Serum concentration of each marker was obviously higher in breast cancer stages Ⅲ+Ⅳgroup and postoperative recurrence group than in breast benign tumor group and breast cancer stagesⅠ+Ⅱ group. Positive diagnosis rate in breast cancer stagesⅠ+Ⅱ group by single or combined detec－tion was not high. Some commonly used combination detections were compared and analyzed and the combined detection of CYFRA21-1 and CA72-4 was of great significance，which can achieve combined effect of five indexes. CA15-3，CA125，CYFRA21-1，CA72-4 and TAM concentrations were significantly reduced in effective group than in before chemotherapy group and invalid group，with sig－nificant statistical differences（P<0.01）. Conclusions：Combined detection of CYFRA21-1 and CA72-4 is complementary and is great of value in monitoring postoperative relapse and metastasis. Detections of serum CA125，CA15-3，CYFRA21-1，CA72-4 and TAM are of great help for chemotherapy drug efficacy evaluation.
