半胱氨酸蛋白酶-12表达与细胞色素C释放在脊髓压迫性损伤后少突胶质细胞凋亡中的作用

Role of Caspase-12 expression and cytochrome-C release in oligodendrocyte apoptosis induced by compressed spinal cord injury

摘要:

目的：探讨半胱氨酸蛋白酶-12（Caspase-12）表达与细胞色素C（cytochrome-C，Cyto-C）释放在脊髓压迫性损伤（com－pressed spinal cord injury，CSCI）后少突胶质细胞凋亡中的作用。方法：采用自行设计的方法制作脊髓压迫模型，通过原位细胞凋亡染色于压迫后1、3、7 d检测CSCI后少突胶质细胞凋亡；运用Western blot技术从分子水平分别检测与细胞凋亡有关的Caspase-12和Cyto-C的表达。结果：不同组别凋亡的少突胶质细胞数目不全相同（F=30.946，P=0.000），LSD-t法两两比较，1、3、7 d模型组凋亡的少突胶质细胞数目均高于正常组（P=0.000）。与对照组、1 d组、3 d组相比，7 d组凋亡的少突胶质细胞数目最多，组间比较，差异有统计学意义（P=0.000）。不同组别Caspase-12和Cyto-C蛋白表达不全相同（F分别等于1 402.646和326.638，P=0.000），LSD-t法两两比较，1、3、7 d模型组的Caspase-12和Cyto-C蛋白表达均高于正常组（P=0.000），与对照组、1 d组、3 d组相比，7 d组的Caspase-12和Cyto-C蛋白表达水平均最高，组间比较，差异有统计学意义（P=0.000）。结论：CSCI后出现了少突胶质细胞凋亡，其凋亡机制可能与Caspase-12表达增强及Cyto-C释放共同作用有关。

Abstract:

Objective：To evaluate the role of Caspase-12 expression and cytochrome-C（Cyto-C） release in oligodendrocyte apoptosis induced by compressed spinal cord injury（CSCI）. Methods：CSCI model was established with a self-made device. Oligodendrocyte apoptosis was determined by TdT mediated dUTP-biotin nick end labeling staining at 1，3，7 d after compression. Cyto-C release and Caspase-12 expressions were detected by Western blot. Results：Numbers of oligodendrocyte apoptosis in different groups were differ－ent（F=30.946，P=0.000）. Numbers of oligodendrocyte apoptosis in 1，3，7 d groups were more than those in control group according to LSD-t（P=0.000）. Numbers of oligodendrocyte apoptosis in 7 d group reached the maximum compared with those in control group，1 d group and 3 d group，with statistically significant differences among groups（P=0.000）. Both expressions of Caspase-12 and releases of Cyto-C in different groups were different（F=1 402.646 and 326.638 respectively，P=0.000）. Expressions of Caspase-12 and releas－es of Cyto-C in 1，3，7 d groups were more than those in control group according to LSD-t（P=0.000）. Expressions of Caspase-12 and releases of Cyto-C in 7 d group reached the maximum compared with those in control group，1 d group and 3 d group，with statistically significant differences among groups（P=0.000）. Conclusions：Oligodendrocyte apoptosis after CSCI is associated with enhanced expressions of Caspase-12 and releases of Cyto-C.