养血清脑颗粒对缺血/再灌注大鼠脑炎性因子表达和轴突再生的影响

Effects of Yangxueqingnao granule on inflammatory factor expressions and axonal regeneration in rats with focal cerebral ischemia reperfusion

摘要:

目的：探讨养血清脑颗粒对大鼠局灶性脑缺血/再灌注损伤后皮质核因子-κBp65（nuclear factor-κBp65，NF-κBp65）、白细胞介素-6（interleukin-6，IL-6）、神经微丝蛋白-200（neurofilament protein-200，NF-200）的表达及神经功能的影响。方法：72只健康的成年雄性SD大鼠，随机分为假手术组（S组）、缺血/再灌注组（I/R组），生理盐水对照组（I/R+C组）和养血清脑颗粒治疗组（I/R+YXQ组）。在缺血/再灌注后第2天及第2周，分别采用免疫组织化学法观察NF-κBp65，IL-6，NF-200的表达，West－ern blot法检测缺血脑组织NF-κBp65蛋白的表达，楼梯测试评估大鼠的神经功能。结果：缺血/再灌注后第2天，I/R组大鼠缺血侧皮质NF-κBp65，IL-6表达明显增高（P<0.001），NF-200表达显著降低（P<0.001），同I/R组相比，I/R+YXQ组NF-κBp65，IL-6表达降低（P<0.001）；缺血/再灌注后第2周，同I/R组相比，I/R+YXQ组NF-200表达增加（P<0.001），神经功能评分无明显改善（P=0.178）。结论：养血清脑颗粒可能通过减轻局灶性脑缺血/再灌注损伤后皮质神经元的炎症反应，从而促进轴突相关蛋白的表达，改善神经功能。

Abstract:

Objective：To investigate the effects of Yangxueqingnao granule on expressions of nuclear factor-κBp65（NF-κBp65），in－terleukin-6（IL-6） and neurofilament protein-200（NF-200） in cortex and neurological function after focal cerebral ischemia reperfu－sion injury in rats. Methods:Seventy-two SD rats were randomly divided into sham-operated group（S group），cerebral ischemia/reperfusion group（I/R group），normal saline control group（I/R+C group） and Yangxueqingnao granule treated group（I/R+YXQ group）. Neurological function was measured using Montoya staircase test. Changes in expressions of NF-κBp65 and IL-6 were analyzed by immunohistochemistry and Western blot，respectively. NF-200 immunohistochemical staining was used to evaluate axonal regener－ation. Results：At the 2nd d after ischemia reperfusion，expressions of NF-κBp65 and IL-6 in ischemic cortex were significantly in－creased（P<0.001），while those of NF-200 were significantly decreased in I/R group（P<0.001）. Expressions of NF-κBp65 and IL-6 were lower in IR+YXQ group than in I/R group（P<0.001）. At the 2nd week after ischemia reperfusion，expressions of NF-200 positive cells in I/R+YXQ group were increased compared with those in the I/R group（P<0.001） and behavioral tests showed no difference in neurological improvement（P=0.178）. Conclusions：Yangxueqingnao granule can improve neurological function recovery after focal cerebral ischemic reperfusion injury in rats，which may be associated with the inhibition of inflammation reactions.