癫痫大鼠模型海马组织突触素-1的表达变化研究

Changes in expressions of synaptophysin-1 in the hippocampus of rats in amygdala-kindling epilepsy model

摘要:

目的：探讨杏仁核点燃癫痫发生过程中突触素-1（synaptophysin，SYN-1）在海马表达动态变化。方法：建立杏仁核点燃癫痫大鼠模型及左乙拉西坦（levetriacetam，LEV）治疗模型，用real time-PCR测定不同时期SYN的表达量，并测量免疫反应光密度值。结果：SYN-1见于癫痫1周组阳性细胞率最低，表达最少，8周组表达最高，差异均有统计学意义（P<0.05），并且8周组用药前后比较有统计学差异，大鼠在14 d开始出现自发发作。结论：SYN-1与突触形成与重建密切相关，在癫痫组亚组8周组表达上调，提示其可能在慢性期参与了癫痫的维持。

Abstract:

Objective：To establish the amygdala-kindling epilepsy rat model，to measure the expression of synaptophysin-1（SYN-1) and to explore the pathogenesis of epilepsy. Methods：Totally 140 male Wistar rats were randomly divided into epilepsy group（n=60），drug treatment group（n=60） and sham operation group（n=20）. Amygdala-kindling epilepsy model and levetiracetam treatment model were established. Expressions of SYN-1 at different time points were determined by real-time PCR and absorbance of immunoreaction was detected. Results：Expression of SYN-1and positive cell rate was the lowest in epilepsy-one-week group and was the highest in epilepsy-eight-week group，with statistical differences among groups（P<0.05）. There were statistical difference in epilepsy-eight-week group before and after drug treatment；spontaneous seizure was observed in rats at 14 d after drug treatment. Conclusions：SYN-1 is closely related with synaptic formation and reconstruction. Expression of SYN-1 is higher in epilepsy-eight-week group than in sham operation group，suggesting that SYN- 1 may be involved in the maintenance of epilepsy at chronic stage.