载脂蛋白E基因启动子区多态性与蛛网膜下腔出血后脑血管痉挛的相关研究

Correlation between apolipoprotein E gene promoter polymorphisms and cerebral vasospasm after spontaneous subarachnoid hemorrhage

摘要:

目的：探讨载脂蛋白E基因（apolipoprotein E，APOE）启动子区多态性与自发性蛛网膜下腔出血（subarachnoid hemor－rhage，SAH）后脑血管痉挛（cerebral vasospasm，CVS）的相关性。方法：采用聚合酶链反应限制性片段长度多态性检测101例SAH患者，测定APOE启动子区多态性；采用经颅多普勒超声评定SAH患者出血后的CVS程度；对APOE启动子区多态性与CVS等临床资料分别进行χ2检验和Logistic回归分析。结果：101例SAH患者中，－219T等位基因携带者的CVS发生率显著高于－219G等位基因携带者（P＝0.024）；单因素和多因素Logistic回归分析发现，－219T是发生CVS的危险因素。结论：APOE启动子区－219T等位基因是SAH患者发生CVS的危险因素。

Abstract:

Objective：To investigate the correlation between apolipoprotein E gene（APOE） promoter polymorphisms and cerebral vasospasm（CVS） after spontaneous subarachnoid hemorrhage（SAH）. Methods：One hundred and one patients with spontaneous SAH were selected in this study. Venous blood samples and clinical data of patients were collected. APOE promoter polymorphisms of all patients were determined by polymerase chain reaction-restriction fragment length polymorphism. Degrees of CVS after SAH were de－termined by transcranial Doppler. APOE promoter polymorphisms and clinical data of CVS after SAH were analyzed by χ2 test and Logistic regression. Results：In all patients，incidence of CVS in -219T group was significantly higher than that in -219G group（P=0.024）. Univariate and multivariate Logistic regression analyses also showed that promoter -219T was a risk factor of CVS incidence. Conclusion：-219T alleles in APOE promoter region is the risk factor of CVS after spontaneous SAH.