急性脑梗死患者不同病情程度与血清中PAC-1和CD62p的不同表达分析

Analysis on expressions of platelet activator combined-1 and P-selectin in serum of patients with acute cerebral infarction at different degrees

摘要:

目的：探讨不同病情程度急性脑梗死（acute cerebral infarction，ACI）患者血小板膜糖蛋白纤维蛋白原受体（platelet ac－tivator combined-1，PAC-1）和P选择素（也称CD62p）的表达情况。方法：分析我院自2008年10月至2011年10月收治的ACI患者287例，根据脑梗死病灶大小分为腔隙性脑梗死组76例、小面积脑梗死组125例和大面积脑梗死组86例，同时选取50例健康人群为对照组。采用流式细胞仪检测外周血PAC-1和CD62p含量。结果：ACI组PAC－1及CD62p水平（46.9 mg/L，28.4 ng/L）与对照组（29.6 mg/L，10.3 ng/L）比较均上升，差异有统计学意义（t=2.042，P=0.044；t=2.137，P=0.038）。腔隙性脑梗死组、小面积脑梗死和大面积脑梗死组在PAC－1及CD62p水平比较，差异有统计学意义（F=7.32，P=0.046；F=5.38，P=0.043）。小面积脑梗死组的PAC－1及CD62p水平（39.9 mg/L，24.5 ng/L）与腔隙脑梗死组（45.9 mg/L，29.1 ng/L）比较，差异有统计学意义（SNK-q＝4.417、4.129；P＝0.031、0.037）。大面积脑梗死组（53.9 mg/L，33.8 ng/L）与小面积脑梗死组、腔隙脑梗死组的PAC－1及CD62p水平比较，差异有统计学意义（SNK-q＝4.216、4.022；P=0.039、0.042）。结论：PAC-1 和CD62p 在ACI 患者中显著表达且可用于ACI病情严重程度的检测，临床检测对于预防和监控疾病发展具有重大意义。

Abstract:

Objective：To explore expressions of platelet activator combined-1（PAC-1） and P-selectin（CD62p） in patients with acute cerebral infarction（ACI） at different degrees. Methods：Totally 287 patients with ACI from October 2008 to October 2011 were divid－ed into lacunar infarction group（n=76），small volume ACI group（n=125） and large volume ACI group（n=86）；50 health volunteer were served as control group. Levels of PAC-1 and CD62p were detected by flow cytometry. Results：Levels of PAC-1 and CD62p were increased in ACI group（46.9 mg/L，28.4 ng/L） than in control group（29.6 mg/L，10.3 ng/L）with significant differences（t=2.042，P=0.044；t=2.137，P=0.038）. Levels of PAC-1 and CD62p were significantly different among three groups（F=7.32，P=0.046；F=5.38，P=0.043）. Levels of PAC-1 and CD62p were significantly different between small volume ACI group（39.9 mg/L，24.5 ng/L） and la－cunar infarction group（45.9 mg/L，29.1 ng/L）（SNK-q＝4.417，4.129；P＝0.031，0.037）. Levels of PAC-1 and CD62p were significantly different between large volume ACI group（53.9 mg/L，33.8 ng/L） and small volume ACI group and lacunar infarction group（SNK-q＝4.216，4.022；P=0.039，0.042）. Conclusions：Significant expressions of PAC-1 and CD62p in patients with ACI can be used to detect the degrees of ACI and are of great importance in preventing and monitoring diseases development.