冷保存/再灌注损伤对大鼠移植肝术后胆汁酸代谢轮廓的影响

Effects of cold preservation reperfusion injury on the metabolite profiling of bile acids in rats after liver transplantation

摘要:

目的：探讨大鼠移植肝脏经历冷保存/再灌注损伤（cold preservation reperfusion injury，CPRI）后早期胆汁酸的变化规律。方法：采用柱前衍生反相高效液相色谱荧光检测法对供肝冷保存 12 h组、1 h组和对照组大鼠术后胆汁中胆汁酸成分进行测定，并结合主成分分析法和偏最小二乘判别分析法对胆汁酸代谢轮廓进行分析。结果：胆汁中胆汁酸代谢轮廓可区分 CPRI 12 h组、1 h组和对照组，牛磺脱氧胆酸与胆管损伤密切相关且肝移植术后 CPRI 12 h组的疏水指数明显增高，并与供肝冷保存时间呈正相关。结论：CPRI影响肝移植大鼠胆汁中胆汁酸代谢轮廓。

Abstract:

Objective：To investigate the effects of cold preservation reperfusion injury（CPRI） on the variation of bile acid composition in rats after liver transplantation. Methods：Reverse phase-high performance liquid chromatography with precolumn derivatization and fluorescence detection was used to determinate the bile acid composition in rat bile after the surgery by using the donor livers which were coldly preserved for 12 h，1 h and the control livers. Metabolite profiling analysis of bile acid was performed by the methods of principal component analysis and partial least squares-discriminant analysis. Results：It was obvious that CPRI for 12 h，1 h groups and the control group can be separated with the metabolite profile of bile acids and bile duct injury was probably closely related with taurodeoxycholic acid. Hydrophobicity index of CPRI for 12 h group was increased significantly after liver transplantation and was closely correlated with cold preservation time of the donor livers. Conclusion：Metabolite profiling of bile acids in rat bile af－ter liver transplantation is significantly influenced by CPRI.