肿瘤侵犯相关蛋白-自分泌运动因子对真皮成纤维细胞生物学特性的影响

Effects of tumor invasion related protein-autocrine motility factor on biological property in human dermal fibroblasts

摘要:

目的：观察自分泌运动因子（autocrine motility factor，AMF）对体外培养的人真皮成纤维细胞（human dermal fibroblasts，ＨＤＦ）生物学特性（增殖、抗凋亡和迁移运动）的影响。方法：HDF体外培养后分为AMF组和空白对照组。MTT法检测细胞增殖活性，流式细胞仪检测细胞周期及细胞抗凋亡能力，Transwell小室迁移运动模型检测细胞迁移运动能力。结果：与空白对照组相比，AMF组体外培养的HDF的增殖能力（t=9.13，P＜0.001）、抗凋亡能力（t=30.94，P＜0.001）及运动迁移能力（t=5.35，P=0.003）均显著增强。结论：AMF具有提高HDF增殖、运动迁移和抗凋亡能力，提示该因子与瘢痕疙瘩的肿瘤学特征有着密切关系。

Abstract:

Objective：To observe the effects of autocrine motility factor（AMF） on the biological property（proliferation，anti-apoptosis and migration）of the cultured human dermal fibroblasts（HDF）. Methods：Cultured HDF were divided into experimental group（AMF group） and blank control group. Cellular anti-apoptosis and cell cycle were analyzed by flow cytometry. Proliferation and migration of the cells were respectively determined by MTT assay and Transwell chamber. Results：Abilities of proliferation（t=9.13，P<0.001），an－ti-apoptosis（t=30.94，P<0.001） and migration（t=5.35，P=0.003） were increased significantly in experimental group compared with those in blank control group. Conclusions：AMF could induce abilities of proliferation，anti-apoptosis and migration of HDF，suggest－ing that AMF is associated with keloid in characteristics of oncology.