穴位电针对功能矫治大鼠咀嚼肌基质金属蛋白酶－9表达的影响

Effects of acupoints electroacupuncture on the expression of matrix metalloproteinase-9 in the mandibular protrusion of rats

摘要:

目的：研究穴位电针对大鼠下颌前伸后咀嚼肌中基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）表达的影响。方法：60只雄性SD大鼠随机分为12组（n=5）：空白对照3、7、14、21 d组，条件对照3、7、14、21 d组和实验3、7、14、21 d组。采用免疫组化染色法和荧光定量PCR法检测大鼠二腹肌前腹中MMP-9表达的差异。结果：与空白对照组比较，条件对照组在7、14 d时MMP-9表达明显上升（P<0.05），实验组在3、7、14 d时MMP-9的表达明显上升（P<0.05）；与条件对照组比较，实验组在3、7 d时MMP-9的表达明显上升（P<0.05），14 d时两组无明显差异（P >0.05），实验组21 d MMP-9表达量降低（P<0.05）。结论：穴位电针可早期上调大鼠下颌前伸咀嚼肌中MMP-9的表达，促进大鼠咀嚼肌的适应性改建。

Abstract:

Objective：To investgate effects of acupoints electroacupuncture on the expression of matrix metalloproteinase-9 （MMP-9）in the mandibular protrusion of rats. Methods：Totally 60 male SD rats were randomly divided into 4 blank control groups，4 con－ditional control groups and 4 experimental groups（n=5，respectively）. Rats were sacrificed on 3，7，14，28 d after experimental mandi-bular protrusion. Immunohistochemistry and real-time PCR methods were used to detect expressions of MMP-9 in growing rats’ mas－ticatory muscle. Results：Compared with those in blank control groups，MMP-9 expressions were significantly increased in conditional control group on 7，14 d，with statistical differences（P<0.05）；MMP-9 expressions were significantly increased in experimental group on 3，7，14 d，with statistical differences（P<0.05）. Compared with those in conditional control group，MMP-9 expressions were signifi－cantly increased in experimental group on 3，7 d，with statistical differences（P<0.05）；there was no significant difference between the two groups on 14 d（P>0.05）；MMP-9 expressions were significantly decreased in experimental group on 21 d，with statistical differ－ences（P<0.05）. Conclusions：Electric acupuncture can up regulate the expression of MMP-9 in rats masticatory muscle after func－tional mandibular protrusion，which may improve the treatment effect of functional appliance.