改良LPD液、硝普钠对犬肺缺血/再灌注损伤内皮素-1的影响

Effects of modified low potassium dextran solution and sodium nitroprusside on endothelin-1 during ischemia-reperfusion injury in dogs

摘要:

目的：采用犬单肺原位常温缺血/再灌注模型，探讨及研究改良LPD液（low potassium dextra glucose，LPD），改良LPD液+硝普钠2种保护液对犬肺缺血/再灌注损伤时血浆及肺组织匀浆中内皮素-1（endothelin-1，ET-1）的影响有无差异。方法：选用健康成年杂交犬12只，随机分成2组，每组6只，A组以常温LPD液行左肺灌洗，灌注量50～60 ml/kg，灌注压力< 40 mmH2O；B组以常温LPD液+硝普钠行左肺灌洗，其余同A组。2组采用酶联免疫吸附法分别于再灌注后0、2、4 h测定血浆、肺组织匀浆中ET-1的含量以及实验结束时称取肺湿、干重，计算肺湿/干比重。结果：2组血浆、肺组织匀浆中的ET-1含量无差异（P >0.05），肺湿/干比重比较有变化（P<0.05）。结论：改良的LPD液可能通过减少ET-1的表达水平，降低肺湿/干比重，减轻肺缺血/再灌注损伤，但在改良的LPD液基础上加上硝普钠，对ET-1的表达水平及肺湿/干比重无明显影响。

Abstract:

Objective：To study effects of modified low potassium dextran（LPD） solution combined with sodium nitroprusside on en－dothelin-1（ET-1） in plasma and lung homogenate during ischemia-reperfusion injury of dogs. Methods：A total of 12 healthy adult hybrid dogs were randomly divided into two groups with six dogs in each group. In group A，left lungs were flushed with 50-60 ml/kg of hepatic modified LPD solution at a pressure of less than 40 mmH2O. In group B，left lungs were flushed with hepatic modified LPD solution and sodium nitroprusside with other measures the same as group A. Changes of ET-1 content in plasma and lung homogenate at 0，2 h and 4 h after reperfusion were observed by ELISA. At the end of the experiment，the variation of lung wet/dry weight ratio was calculated. Results：ET-1 contents in plasma and lung homogenate were not significantly different（P >0.05） and wet/dry weight ratio in two groups had significant changes（P<0.05）. Conclusions：Modified LPD solution can reduce ET-1 expression level and wet/dry weight ratio and relieve ischemia-reperfusion injury，however，modified LPD solution combined with sodium nitroprusside has no effect on ET-1 expression level and wet/dry weight ratio.