果糖对电离辐射诱发AHH-1细胞凋亡的保护作用

Radioprotective effects of fructose on irradiation induced apoptosis in AHH-1 cells

摘要:

目的：观察果糖对淋巴母细胞AHH-1辐射损伤保护作用。方法：照射前12 h给予不同浓度的腺嘌呤，再经4.0 Gy 60Coγ射线照射诱发细胞凋亡，采用MTS法计算细胞存活率；Annexin V-FITC/PI双染法检测细胞凋亡率；RT-PCR分析细胞Caspase-9、Bax、Bcl-2 mRNA表达的变化；Western blot 分析细胞Caspase-9、Bax、Bcl-2蛋白改变。结果：与正常组相比，电离辐射后细胞存活率78.0%显著下降（P<0.05），细胞照射前加入果糖其存活率随浓度的增加而升高（P<0.05），细胞中Caspase-9、Bax的mRNA和蛋白表达明显减少，Bcl-2的mRNA和蛋白表达明显增加，凋亡的各项指标均明显改善。结论：果糖对电离辐射诱发的细胞凋亡有保护作用，并对细胞凋亡的抑制呈剂量依赖正相关关系。

Abstract:

Objective：To investigate radioprotective effects of fructose on irradiated lymphocytoblast AHH-1 and to discover the possi－ble mechanisms. Methods：Lymphocytoblasts were pretreated for 12 h before radiation with different concentrations of fructose and then were exposed to 4 Gy 60Coγ ray radiation to induce cell apoptosis. Cell survival rate was counted by MTS assay and cell apoptosis rate was detected by Annexin V/PI. Changes in mRNA expressions of Caspase-9，Bax，Bcl-2 were investigated by RT-PCR and changes in protein expressions of Caspase-9，Bax，Bcl-2 were analyzed by Western blot. Results：Irradiation increased cell death and cell survival rate was 78% after irradiation，significantly lower compared with that before irradiation（P<0.05）. If fructose was added before irradiation，cell survival rate was increased in a concentration dependent manner after irradiation（P<0.05）. mRNA and proteins expressions of Caspase-9 and Bax were significantly decreased and mRNA expressions of Bcl-2 were increased；apotososis related indexes were improved. Conclusions：Fructose exerts radioprotective effects on irradiation induced apoptosis in AHH-1 cells and its inhibition on cell apoptosis is in a concentration dependent manner.