基质金属蛋白酶-9和基质金属蛋白酶组织抑制物-3在反复体外受精-胚胎移植助孕失败患者子宫内膜中的表达

Expressions of matrix metalloproteinase -9 and tissue inhibitor of metalloproteinase-3 in the endometrium of patients experiencing recurrent in vitro fertilization-embryo transfer failure

摘要:

目的：检测基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）和基质金属蛋白酶组织抑制物-3（tissue inhibitor of metalloproteinase-3，TIMP-3）在反复体外受精-胚胎移植（in vitro fertilization-embryo transfer，IVF-ET）助孕失败患者子宫内膜中的表达，并探讨MMP-9、TIMP-3与胚胎着床的关系及可能机制。方法：应用免疫组织化学SP法，检测40例反复IVF-ET助孕（≥3次）失败者（实验组）及40例经1次IVF-ET助孕即妊娠者（对照组）的黄体中期内膜MMP-9和TIMP-3表达。结果：MMP-9在实验组的表达明显低于对照组，差异有统计学意义（P<0.05）；而TIMP-3在两组间表达的差异不具有统计学意义（P >0.05）。结论：MMP-9在黄体中期子宫内膜的表达降低可能会通过影响胚胎着床而导致反复IVF-ET助孕失败的发生。

Abstract:

Objective：To detect expressions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-3（MMP-9/TIMP-3） in the endometrium of patients experiencing recurrent in vitro fertilization-embryo transfer（IVF-ET） failure and to explore the possi－ble mechanism. Methods：Expressions of MMP-9/TIMP-3 in endometrium at mid-luteal phase in 40 patients experiencing recurrent IVF-ET failure（experiment group） and 40 pregnant women with IVF-ET once a time（control group） were detected by immunohisto－chemistry SP. Results：Expressions of MMP-9 in experimental group were significantly lower than those of control group（P<0.05），but there was no significant difference in TIMP-3 expressions between both groups（P >0.05）. Conclusions：Recurren IVF-ET failure is possibly caused by lower expressions of MMP-9 in endometrium at mid-luteal phase.