波特兰水门汀对3种微生物的体外生长抑制实验研究

Antimicrobial effects of portland cement against three different microorganisms： an in vitro study

摘要:

目的：比较波特兰水门汀（portland cement，PC）、无机三氧化物聚合物（mineral trioxide aggregate，MTA）、玻璃离子水门汀（glass ionomer cement，GIC）对3种微生物的体外生长抑制能力。方法：采用琼脂扩散法检测3种材料对3种微生物的体外生长抑菌效果。将含有1.5×108 CFU/ml活菌的白色念珠菌、大肠杆菌、变形链球菌菌悬液分别接种于不同的琼脂平板，将新鲜调拌的直径5 mm、厚度1 mm的材料片等间距放入琼脂平板内，37 ℃恒温培养24～48 h后，测量材料周围的抑菌环直径大小，根据抑菌环直径大小评价3种材料的体外细菌生长抑制能力。结果：研究发现3种材料对变形链球菌均无抑菌环产生；GIC对3种微生物均无抑菌环产生；PC及MTA对白色念珠菌均有抑菌效果，且PC产生的抑菌环直径大于MTA，差异具有统计学意义（P=0.002 9）；PC及MTA对大肠杆菌均有抑菌效果。结论：PC对白色念珠菌及大肠杆菌有一定抑制生长能力，可考虑进一步深入研究，为其临床应用提供理论支持。

Abstract:

Objective：To evaluate the antimicrobial activity of portland cement（PC），mineral trioxide aggregate（MTA） and glass ionomer cement（GIC）against three microorganisms：candida albicans，escherichia coli，streptococcus mutans through the Agar diffusion method. Methods：The sealers under study were handled according to the manufacturer’s instruction. All sealers were handled with diameter of 5 mm and thickness of 1 mm then placed in the wells which contains 1.5×108 CFU/mL microorganisms suspension of candida albi－cans，escherichia coli and streptococcus mutans，respectively immediately after being mixed. After the sealers set，the agar plates were incubated at 37 ℃ for 24~48 h to allow the microorganisms to grow and reagents to diffuse through the culture medium. After incuba－tion，diameter of inhibition zones was measured. Results：No antimicrobial activity of all the three materials was observed on strepto－coccus mutans. No inhibition zone of GIC was observed on the three microorganisms. Diameter of inhibition zone of PC on candida al－bicans was bigger than that of MTA with significant difference between two groups（P=0.002 9）. Conclusions：PC could effectively in－hibit the growth of candida albicans and escherichia coli，therefore PC is considered to have certain antifungal and antibacterial prop－erties.