核转录因子-κB和缺氧诱导因子-1α在前列腺癌中的表达及意义

Expressions and clinical significances of nuclear factor-κB and hypoxia inducible factor-1α in prostate cancer

摘要:

目的：研究核转录因子-κB（nuclear factor kappa B，NF-κB）和缺氧诱导因子-1α（hypoxia inducible factor-1α，HIF-1α）在前列腺癌中的表达及其临床意义。方法：采用逆转录聚合酶链反应（reverse transcriptase-polymerase chain reaction，RT-PCR）和免疫组织化学光谱纯试剂（spectrum pure，SP）法检测62例前列腺癌组织及17例良性前列腺增生组织中NF-κB和HIF-1α的mRNA和蛋白的表达。结果：（１）RT-PCR结果显示NF-κB和HIF-1α的mRNA在前列腺癌组中的相对表达量明显高于前列腺增生（P＜0.05）。免疫组织化学结果显示NF-κB和HIF-1α蛋白在前列腺癌组中的阳性表达率分别为62.90％和66.13％，明显高于前列腺增生组11.77％和5.88％（P＜0.05）。（２）NF-κB和HIF-1α的阳性表达均与前列腺癌的Gleason分级、临床分期有关（P＜0.05），而与年龄无关（P ＞0.05）。（３）NF-κB和HIF-1α的表达呈正相关（rs=0.368，P＜0.05）。结论：NF-κB和HIF-1α可能与前列腺癌的发生、发展密切相关，并可能在前列腺癌的浸润和转移中起重要作用。

Abstract:

Objective：To study expressions and clinical significances of nuclear factor-κB（NF-κB） and hypoxia inducible factor-1α （HIF-1α） in prostate cancer. Methods：RT-PCR and immunohistochemistry SP method were used to detect NF-κB and HIF-1α mRNA and protein expressions in 62 cases of prostate cancer tissues and 17 cases of prostatic hyperplasia tissues. Results：（１）RT-PCR results showed that NF-κB and HIF-1α mRNA expression levels were relatively significantly higher in prostate cancer group than in prostatic hyperplasia group，with statistical differences（P<0.05）. Immunohistochemical results showed that positive expression rates of NF-κB and HIF-1α proteins in prostate cancer group were 62.90% and 66.13%，8.33% and 5.88%，significantly higher than those in prostatic hyperplasia group with statistically significant differences（P<0.05）.（２）Expressions of NF-κB and HIF-1α were correlated with Gleason grade and clinical stages of prostate cancer（P<0.05），but not with age（P >0.05）. （３）Expressions of NF-κB and HIF-1α were positively correlated（rs=0.368，P<0.05）. Conclusions：Overexpression of NF-κB and HIF-1α may be involved in the pathogene－sis and progression of prostate cancer.Moreover，NF-κB and HIF-1α may be correlated with the invasion and metastasis of prostate cancer.