卵巢上皮性肿瘤fibulin-5表达与血管生成的关系研究

Expressions of fibulin-5 and their relationship with angiogenesis in epithelial ovarian tumor

摘要:

目的：探讨卵巢上皮性肿瘤抗衰老关键蛋白（fibulin-5）表达与血管生成的关系。方法：术前应用彩色多普勒超声检测卵巢肿瘤血流并计测阻力指数（resistance index，RI），术后采用免疫组织化学SP法检测89例卵巢上皮性肿瘤组织fibulin-5及CD34蛋白表达情况，并以CD34标记的血管内皮细胞计数肿瘤组织微血管密度（microvessel density，MVD），结合临床病理参数分析卵巢癌fibulin-5表达与肿瘤生物学行为的关系，并对fibulin-5表达、MVD及RI进行相关性分析。结果：fibulin-5阳性表达在良性、交界性、恶性卵巢上皮性肿瘤间比较差别有统计学意义（ ?字2=20.654，P=0.000）。良性﹑交界性﹑恶性卵巢上皮性肿瘤MVD呈上升趋势，且任两组间比较差别均有统计学意义（F=88.97，P=0.000）。卵巢癌RI中位数显著低于良性卵巢上皮性肿瘤（ ?字2=12.200，P=0.002），且与MVD呈负相关（rs=-0.676，P=0.000）。卵巢癌fibulin-5阳性表达在不同临床分期间比较差别有统计学意义，但在不同年龄、分化程度及病理类型间比较差别无统计学意义，且fibulin-5阳性表达与MVD呈负相关（rs=-0.650，P=0.000），与RI呈正相关（rs=0.430，P=0.004）。结论：fibulin-5随卵巢上皮性肿瘤进展而表达降低且与肿瘤血管生成相关。

Abstract:

Objective：To investigate expressions of fibulin-5 and their relationship with angiogenesis in epithelial ovarian tumor. Methods：Blood flow signals of ovarian tumor were detected preoperatively by color Doppler ultrasound and resistance index（RI） was used for analysis. Expressions of fibulin-5 and CD34 in 89 patients with epithelial ovarian tumor were assessed by immunohistochemi－cal SP method after operation. Microvessel density（MVD） was counted with endothelial cells marked by CD34.Relationship between expressions of fibulin-5 and tumor biological behavior was analyzed by MVD combined with clinical pathological parameters. Correla－tion among fibulin-5，MVD and RI in epithelial ovarian carcinoma was analyzed． Results：There were significant differences in positive expression of fibulin-5 among benign，border and malignant epithelial ovarian tumors（ ?字2=20.654，P=0.000）. MVD among benign，bor－der and malignant tumors showed ascendant trend and there were significant differences between groups（F=88.97，P=0.000）. Median of RI was significantly lower in ovarian carcinoma than in benign epithelial ovarian tumor（ ?字2=12.200，P=0.002） and it was negatively correlated with MVD（rs=-0.676，P=0.000）. There were statistical significances in positive expressions of fibulin-5 among different clinical stages in ovarian carcinoma，but there was no difference among different age，differentiation degrees and pathological types. Positive expressions of fibulin-5 in ovarian carcinomas were negatively correlated with MVD（rs=-0.650，P=0.000） and were positively correlated with RI（rs=0.430，P=0.004）. Conclusions：Expression of fibulin-5 protein is reduced with the progression of epithelial ovari－an tumor and it is correlated with the tumor angiogenesis.