老年高血压患者克老素基因单核苷酸多态性与其左心室肥厚的相关性研究

Association of single nucleotide polymorphism of three sites of klotho gene with left ventricle hypertrophy in elderly hypertension patients

摘要:

目的：研究老年高血压人群克老素（klotho，KL）基因3个位点单核苷酸多态性（single nucleotide polymorphism，SNP）与其左心室肥厚（left ventricle hypertrophy，LVH）的相关性。方法：选择362例老年高血压患者作为研究对象，提取外周血白细胞基因组DNA，应用单一等位基因特异性引物PCR技术检测KL基因3个SNP位点的基因型，同时通过心脏彩超测量相应指标包括左心室舒张末期直径、左心室后壁、室间隔厚度，并测定其身高与体质量等指标。结果：老年高血压病中LVH组KL G-395A SNP GG等位基因频率较无LVH组低（P＜0.05），按性别分层研究发现高血压病女性患者中LVH组KL G-395A SNP GG等位基因频率亦较无LVH组明显降低（P＜0.05）；高血压病LVH组其KL G-395A SNP AA等位基因频率较无LVH组明显增高（P＜0.05），而按性别分层研究却未发现存在性别差异性。高血压病LVH组KLF352V FF、FV、VV及 KLC 370S CC、CS、SS等位基因型频率与无LVH组相比较均无统计学意义（P ＞0.05）。结论：老年高血压患者KL基因G-395A SNP与其LVH有相关性，并发现老年高血压女性患者中GG等位基因携带者可能不易发生LVH。

Abstract:

Objective：To investigate the association of single nucleotide polymorphisms（SNPs） of three sites of klotho（KL） gene with left ventricle hypertrophy（LVH）in the elderly essential hypertension（EH） patients. Methods：Eligible 362 elderly patients with EH were recruited. Genomic DNA of leukocytes was extracted from venous blood and the single allele specific prime PCR technique was used to detect allele genetypes of three SNP sites of KL gene. Left ventricular mass including left ventricular end-diastolic internal dimension（LVED），left ventricular posterior wall thickness（ＬVPWT） and interventricular septal thickness（IVST） were determined by color echocardiogram simultaneously. Height and weight were also detected. Results：Frequency of allele GG genetype of KL G-395A SNP was lower in EH with LVH subgroup than in EH without LVH subgroup（P＜0.05）. According to sex stratification analysis，fre－quency of allele GG genetype was obviously lower in female EH patients with LVH than in female EH patients without LVH（P＜0.05）. Frequency of allele AA genetype of KL G-395A SNP was higher in EH with LVH subgroup than in EH without LVH subgroup（P＜0.05），but it was not significantly different between males and females. There was no difference in frequencies of allele CC，CS and SS genetypes of KL C-370S SNP and allele FF，FV and VV genetypes of KＬ F352V SNP between EH with LVH subgroup and EH without LVH subgroup（P >0.05）. Conclusions：G-395A SNP of KＬ gene is associated with LVH in elderly EH patients and female EH patients carrying GG allelotype of G-395A SNP are less likely to suffer from LVH.