MicroRNA-494通过下调Survivin诱导神经胶质瘤细胞凋亡

Apoptosis of glioma cells induced by microRNA-494 through Survivin down-regulation

摘要:

目的：观察microRNA-494（miR-494）对人神经胶质瘤SNB19和LN229细胞中Survivin的表达调控及对细胞增殖和凋亡的影响。方法：收集12例神经胶质瘤及瘤旁组织，运用qRT-PCR技术检测miR-494在神经胶质瘤及瘤旁组织中的表达情况；应用miR-494 mimic转染人神经胶质瘤细胞系SNB19和LN229，通过qRT-PCR、Western blot、MTT和流式细胞仪检测转染前后Survivin mRNA及蛋白的表达差异以及细胞增殖和细胞凋亡变化。结果：miR-494在神经胶质瘤组织中的表达低于对应的瘤旁组织（t=13.78，P=0.000）；SNB19和LN229细胞株中过表达miR-494明显下调Survivin mRNA及蛋白表达，并导致细胞增殖活力降低和细胞凋亡增加。结论：神经胶质瘤组织中miR-494表达下调，其可能通过下调Survivin表达而诱导神经胶质瘤SNB19和LN229细胞凋亡。

Abstract:

Objective：To investigate the regulation of microRNA-494（miR-494） on Survivin expression and its effect on cell prolifer－ation and apoptosis in human glioma cell lines SNB19 and LN229. Methods：Twelve samples of neuroglioma and tumor-adjacent tissues were collected and miR-494 expressions in the samples were detected by qRT-PCR. miR-494 mimic was transfected into SNB19 and LN229 cells. Cell viability and apoptosis were analyzed by MTT and flow cytometry. Survivin，a potential target of miR-494，was de－tected by qRT-PCR and Western blot. Results：Expressions of miR-494 in neuroglioma tissues were significantly downregulated com－pared with those of tumor-adjacent tissues（t=13.78，P=0.000）. Ectopic expression of miR-494 in SNB19 and LN229 cells obviously suppressed Survivin mRNA and protein expression as well as decreased cell viability and increased cell apoptosis（P<0.05，respective－ly）. Conclusions：Expression of miR-494 is downregulated in neuroglioma tissues and miR-494 may induce cell apoptosis in neu－roglioma cell lines SNB19 and LN229 by downregulating Survivin expression.