三七、黄连、骨碎补凝胶对牙周骨缺损再生疗效的实验研究

Study of the chitosan thermosensitive hydrogel loading Drynaria Rhizome，Coptis chinensis and Panax notoginseng in experimental regeneration of periodontal defect

摘要:

目的：探讨三黄补凝胶对实验性大鼠牙周骨开窗缺损牙周再生修复的作用。方法：选取SD雄性大鼠36只制备下颌骨磨牙区的牙周骨开窗缺损模型，将其配对分组为含三七总皂苷、小檗碱、骨碎补柚皮苷3种中药的壳聚糖凝胶组和不含中药的壳聚糖凝胶组以及空白对照组。分别于术后14 d（n=18）和28 d（n=18）处死大鼠，取术区组织，HE染色，显微镜下观察新生骨形成的情况。结果：术后14 d和28 d载药凝胶组新生牙槽骨面积明显大于空白凝胶组和空白对照组且均有统计学差异（P=0.000）；空白凝胶组新生牙槽骨面积大于空白对照组，差异有统计学意义（P=0.000）。结论：三黄补凝胶能促进牙槽骨的生长，有利于牙周组织的再生修复。

Abstract:

Objective：To discuss the effect of the chitosan thermosensitive hydrogel loading Drynaria Rhizome，Coptis chinensis and Panax notoginseng（sanhuangbu） on the repair of periodontal defects. Methods：Totally 36 male SD rats were enrolled and periodon－tal fenestration model in mandibular molar area was prepared. Then all rats were divided into three groups in matched pair：group filled with chitosan thermosensitive hydrogel loading sanhuangbu（hydrogel and medicine group），group filled with nothing or filled only with chitosan thermosensitive hydrogel（blank hydrogel group）and blank control group. In each group，animals were sacrificed on 14 d（n=18） and 28 d（n=18） postoperatively and the mandibles were processed for histological examination by HE staining. Results：Area of new alveolar bone was extremely lager in hydrogel and medicine group than in blank hydrogel group and blank control group on 14 d and 28 d postoperatively with statistical differences（P=0.000）. Area of new alveolar bone was extremely lager in blank hydrogel group than in blank control group with statistical differences（P=0.000）. Conclusion：Chitosan thermosensitive hydrogel loading sanhuangbu can effectively promote the periodonontal tissue regeneration.