BIRC6在肝细胞癌中的表达及其对肝癌细胞凋亡的影响

Expressions of BIRC6 in hepatocellular carcinoma and their effects on apoptosis of hepatocellular carcinoma cell

摘要:

目的：检测baculoviral IAP repeat-containing 6（BIRC6）在肝细胞癌（hepatocellular carcinoma，HCC）组织及癌旁组织中的表达情况并探讨其在HCC中的功能。方法：收集40例HCC及对应癌旁组织，运用qRT-PCR及免疫组化染色技术检测BIRC6在HCC及对应癌旁组织中的表达情况；qRT-PCR技术检测人正常肝细胞株LO2、HCC细胞株HepG2和SMMC-7721中BIRC6 mRNA表达；应用BIRC6 siRNA转染人HCC细胞SMMC-7721，通过MTT和流式细胞仪检测转染后细胞活力和细胞凋亡变化。结果：BIRC6在HCC组织中的表达高于对应的癌旁组织（P=0.004），HCC组织中BIRC6蛋白高表达与较大肿瘤体积（＞5 cm）和高TNM分期相关（P=0.029；P=0.002）；正常肝细胞株LO2中BIRC6 mRNA表达水平明显低于HCC细胞株HepG2和SMMC-7721（P=0.000），下调BIRC6表达导致SMMC-7721细胞活力降低和细胞凋亡增加（P=0.000）。结论：BIRC6高表达与HCC的恶性临床病理特征相关，BIRC6在HCC中可能作为凋亡抑制因子发挥功能。

Abstract:

Objective：To investigate the expressions of baculoviral IAP repeat-containing 6（BIRC6） and their role in hepatocellular carcinoma（HCC）. Methods：Forty samples of surgically resected HCC tissues and corresponding adjacent tissues were collected and BIRC6 expressions in HCC tissues and corresponding adjacent tissues were detected using qRT-PCR and immunohistochemistry. BIRC6 mRNA expressions in normal liver cell line（LO2） and HCC cell lines（HepG2 and SMMC-7721） were detected using qRT-PCR. BIRC6 siRNA was transfected into SMMC-7721 cells and cell viability and apoptosis were analyzed by MTT and flow cytometry. Results：Expressions of BIRC6 in HCC tissues were significantly upregulated compared with those in corresponding adjacent tissues（P=0.004）. Clinicopathological evaluation suggested that BIRC6 high-expression was associated with large tumor size（＞ 5 cm） and advanced TNM stage（P=0.029；P=0.002）. Expressions of BIRC6 mRNA were obviously lower in LO2 than in HepG2 and SMMC-7721（P=0.000）. siRNA suppressed expression of BIRC6 in SMMC-7721 cells decreased cell viability and increased cell apoptosis（P=0.000）. Conclusions：High-expression of BIRC6 correlates with poor clinicopathological characteristics in HCC and BIRC6 may act as an inhibitor of apoptosis as well as a therapeutic target of HCC.