微量元素锶对1型糖尿病大鼠血清学指标影响的实验研究

Effects of trace element Strontium on serum indexes of rats with type 1 diabetes mellitus

摘要:

目的：观察不同浓度锶矿泉水对1型糖尿病（type 1 diabetes mellitus，T1DM）大鼠的血糖、糖化血红蛋白（glycosylated hemoglobin，ＧHb）、血清甘油三酯（serum triglycerides，TG）、游离脂肪酸（free fatty acid，FFA）、胆固醇（total cholesterol，TC）含量的影响。方法：取健康成年Wistar大鼠150只，先复制T1DM模型（DM鼠）。将DM鼠随机分成A、B、C、D及DM组，分别予以实验方案饲喂处理。各组分别于1、2、3、4月随机处死6只大鼠，采血检测血糖、GHb、TG、FFA、TC含量。结果：第1、2、3、4月，A、B、C、D组及DM组血糖的整体比较无统计学差异（均P >0.05）。第1月，B、C组GHb较DM组显著降低（P<0.05），第2、3、4月，A、B、C、D组与DM组比较差异均无统计学意义。第1、2月，A、B、C、D组血清ＦＦＡ均较DM组明显下降（P<0.05）；第3、4月，D组较DM组明显下降，差异具有统计学意义（P<0.05）。第1月，B、C、D组血清ＴＧ均较DM组明显下降（P<0.05），D组下降最明显（P<0.01）；第2月，D组较DM组明显下降，差异具有统计学意义（P<0.05）；第3、4月，A、B、C、D组与DM组比较无明显差异（P >0.05）。第1月，A、B、C、D组血浆TC与DM组比较差异均无统计学意义（P >0.05）；第2月，D组较DM组明显下降，差异具有统计学意义（P<0.05）；第3、4月，A、B、C、D组与DM组比较均差异无统计学意义（P >0.05）。结论：微量元素锶对T1DM大鼠未显示出明显的降低血糖和ＧＨb的作用；但显示出较好的降低血清FFA、TG和TC的作用。

Abstract:

Objective：To investigate the effects of mineral water containing different concentration of Strontium（Sr） on contents of blood glucose，glycosylated hemoglobin（ＧHb），serum triglycerides（TG），free fatty acid（FFA） and total cholesterol（TC） in rats with type 1 diabetes mellitus（T1DM）. Methods：Totally 150 adult Wistar rats weighing 220~250 g，half male and half female were used in the experiment. One-time intraperitoneal injection of streptozotocin（60 mg/kg） was administrated to rats. Three days later，rats with fasting blood glucose more than 16.7 mmol/L were taken as DM model rats.Then DM rats were randomly divided into group A（drinking mineral water with Sr 2 mg/L），group B（drinking mineral water with Sr 4 mg/L），group C（drinking mineral water with Sr 6 mg/L），group D（rats drinking mineral water with Sr 8 mg/L） and DM group（drinking tap water）. Rats were sacrificed randomly at 1，2，3，4 months later and the indexes mentioned above were detected and compared. Results：There was no significant difference in the overall condition between groups A，B，C，D groups and DM group at the first four months（all P >0.05）. At the first month，blood glucose was decreased in groups B and C than in DM group（P<0.05）；at the second，third and fourth month，there was no statistical difference in blood glucose between groups A，B，C，D and DM group. At the first and second month，FFA was obviously decreased in groups A，B，C，D than in DM group（P<0.05）；at the third and fourth month，FFA was obviously decreased in group D than in DM group（P<0.05）. At the first month，TG was obviously decreased in groups B，C，D（most obviously in group D） than in DM group（P<0.01）；at the second month，TG was obviously decreased in group D than in DM group（P<0.05）；at the third and fourth month，there was no differ－ence in TG between groups A，B，C，D and DM group（P >0.05）. At the first month，There was no significant difference in TC between groups A，B，C，D and DM group（P >0.05）；at the second month，TC was decreased in group D than in DM group（P<0.05）；at the third and fourth month，there was no difference in TC between groups A，B，C，D and DM group（P >0.05）. Conclusions：Race ele－ment strontium exerts no obvious effects on reducing glucose and GHb，but has significant effect on reducing FFA，TG and TC in rats with T1DM.