检测乙肝病毒preS基因常见突变位点的反向斑点杂交技术的建立

Reverse dot blot assay in the detection of mutation sites of hepatitis B virus preS gene

摘要:

目的：利用反向斑点杂交（reverse dot blot，RDB）技术建立用于检测乙肝病毒（hepatitis B virus，HBV）preS基因常见突变位点的方法。方法：根据HBV preS基因变异中的第35、39、48、155、165位氨基酸突变，分别设计寡核苷酸探针，利用RDＢ技术对38例HBV患者突变位点进行检测，并与测序结果进行比较。结果：与测序结果符合率达100%。结论：RDＢ技术可一次性筛查多个不同位点的突变，方便快捷，费用低，对于临床进行HBV preS基因突变检测，判断HBV感染患者的疗效及预后有一定意义。

Abstract:

Objective：To establish a method to detect mutation sites of hepatitis B virus（HBV） preS gene by reverse dot blot（RDB）.Methods：Oligonucleotide probes were designed according to the codon mutations at the 35th，39th，48th，155th and 165th sites，re－spectively. Mutated sites from 38 patients with HBV were detected by RDB and were compared with the sequencing results. Results：Coincidence between RDB detection results and DNA sequencing was 100%. Conclusions：Detection of the preS gene mutation by RDB is a cost-effective，rapid and reliable method，which is of significance in clinical testing for HBV preS mutations and determin－ing the efficacy of HBV infection and prognosis of patients.