云南地区人乳头瘤病毒感染流行病学调查

Epidemiological investigation on human papilloma virus infection in Yunnan province

摘要:

目的：调查人乳头瘤病毒（human papilloma viruses，HPV）在云南地区的感染状况及其分布规律，为云南地区HPV流行病学研究提供依据。方法：运用导流杂交及基因芯片技术对5 376例女性进行21种HPV分型检测，统计HPV在云南地区的感染率及不同HPV型的比例。结果：（１）HPV总感染率为12.8%，21种基因型检出20种，其中单一型别感染率为10.4%，2型混合感染率为1.8%，3型及以上混合感染率为0.6%；（２）HPV52、16型感染最为常见，其次为HPV58、53、33、18，此6种HPV感染占总感染率的65.4%；（３）高危型HPV在≤25岁、26~35岁、36~45岁、≥46岁年龄组的感染率依次为7.4％、13.2％、11.6％、10.3％，组间比较差异具有统计学意义（ χ2=15.33，P <0．01）。低危型HPV在各年龄组的感染率依次为1.2％、2.5％、2.2％和1.3％，组间比较差异无统计学意义（ χ2=6.44，P >0.05）。结论：云南地区HPV感染率低于全国水平（16.1%），以单一亚型感染为主。HPV52、16为高危型HPV感染主要型别，低危型HPV感染以CP8304、HPV11、HPV6 3型常见。HPV各型别的感染率与年龄有一定关系。本研究结果可为宫颈癌的防治和未来抗原肽疫苗的设计提供重要的基础数据。

Abstract:

Objective：To investigate the infection condition and distribution regularity of human papilloma viruses（HPV） in Yunnan province and to provide references for epidemiologic studies of HPV in this district. Methods：Totally 5 376 female subjects were de－tected using flow-through hybridization and gene chip technique for HPV genotyping. Infection rate of HPV and the proportion of different HPV subtypes in Yunnan province were counted. Results：（1）Total infection rate of HPV was 12.8% with 20 out of 21 HPV genotypes being detected. Infection rate of single genotype was 10.4%，of two genotypes was 1.8% and of three or more than three genotypes was 0.6%. （2）The most common HPV genotypes were HPV52 and HPV16，followed by HPV58，53，33 and HPV18. Infec－tion of all of these 6 HPV genotypes accounted for 65.4% of the total infection. （3）Infection rates of high risk HPV genotypes in the age groups of ≤25，26-35，36-45 and ≥46 were 7.4%，13.2%，11.6% and 10.3% respectively，with statistical differences between groups（ χ2=15.33，P <0.01）. Infection rates of low risk HPV genotypes in the age groups mentioned above were 1.2%，2.5%，2.2% and 1.3% respectively，without statistical differences between groups（ χ2=6.44，P >0.05）. Conclusions：HPV infection rate in Yunnan province is lower than the average level of China with single genotype infection being the most common. HPV52 and HPV16 are the high risk HPV genotypes and CP8304，HPV11，HPV6 are the low risk HPV genotypes. Infection rate of each HPV genotype is as－sociated with age. Results obtained in this study can provide thoretical evidence for the prevention and treatment of cervical cancer.