Dicer基因在早孕小鼠子宫内膜中的表达

Expression of Dicer in the endometrium of early pregnant mouse

摘要:

目的：检测Dicer基因在早孕小鼠子宫内膜组织中的表达规律，为阐明其在胚胎着床中的作用打下基础。方法：利用实时荧光定量PCR、免疫组化和Western blot分别检测未孕、假孕及孕d1~d7小鼠子宫内膜中Dicer基因mRNA和蛋白质的表达。结果：在小鼠着床窗期及着床后（d4～d7），小鼠子宫内膜中Dicer基因mRNA和蛋白质表达均明显升高，d5达到高峰（PmRNA=0.000，P蛋白= 0.000）；且Dicer表达主要定位于子宫内膜基质细胞。结论：Dicer基因在早孕小鼠子宫内膜组织中的表达存在时间与空间差异，这种规律性表达所调控的细胞生长、增殖和分化可能是胚胎正常着床的分子机制之一。

Abstract:

Objective：To investigate the expression rules of Dicer in the endometrium of early pregnant mouse and to clarify its role in embryo implantation. Methods：Real-time fluorescence quantitative PCR，immunohistochemistry and Western blot were used to detect the expressions of Dicer mRNA and protein in the mouse endometrium from unpregnant，pseudopregnant and pregnant mouse from d1 to d7. Results：During the implantation window and after the implantation（d4-d7），expression of Dicer mRNA and protein was signif－icantly higher than that before implantation and expression of d5 was the highest（PmRNA=0.000，Pprotein=0.000）. Moreover，Dicer was demonstrated in endometrial stromal cells. Conclusions：There are differences in the time and location of the expression of Dicer in endometrium of early pregnant mouse. Expression rule of Dicer in endometrial stromal cell by regulating cell growth，proliferation and differentiation may be one of the implantation mechanisms.