食物过敏与正常小鼠CD4+CD25+调节性T细胞的差异性研究

Differences of CD4+ CD25+ regulatory T cells between food allergy mice and normal mice

摘要:

目的：比较食物过敏（food allergy，FA）小鼠与正常小鼠模型CD4+CD25+调节性T（regulatory T，Treg）细胞数量及功能差异。方法：4～6周龄SPF级无鸡蛋喂养Balb/c雌鼠随机分为2组，每组8只。分别为卵清蛋白（ovalbumin，OVA）致敏组和正常对照组。采用流式细胞术分析其脾脏单个核细胞悬液中CD4+CD25+ Treg细胞的数量变化；酶联免疫吸附试验（enzyme-linked im－munosorbent assay，ELISA）检测OVA致敏组与正常对照组血清白介素10（interleukin-10，IL-10）和转化生长因子-β1（transform－ing growth factor-β1，TGF-β1）水平。结果：OVA组CD4+CD25+ T淋巴细胞的百分比低于正常对照组（P＜0.05），并且叉状头转录因子3阳性（forkhead transcription factor3+，Foxp3+）CD4+CD25+ Treg细胞的百分比显著低于正常对照组（P＜0.01）；OVA组IL-10低于正常对照组（P＜0.05），TGF-β1水平显著低于正常对照组（P＜0.01）。结论：FA小鼠与正常小鼠调节性T细胞的数量和功能存在差异。

Abstract:

Objective：To compare the quantities and functions of CD4+CD25+ regulatory T（Treg）cells between food allergy mice model and normal mice model. Methods：SPF Balb/c female mice of 4-6 weeks on egg-free diet were randomly divided into ovalbumin（OVA） group（n=8） and normal control group（n=8）. Quantitative changes of CD4+CD25+ Treg cells in the spleen mononuclear cell suspension were analyzed by flow cytometry. Levels of serum interleukin-10（IL-10） and transforming growth factor-β1（TGF-β1） in OVA mice and normal control mice were detected by enzyme-linked immunosorbent assay（ELISA）. Results：Percentage of CD4+CD25+ Treg cells in OVA group was lower than that in normal control group（P<0.05），and percentage of forkhead transcription factor3+（Foxp3+）CD4+CD25+ Treg cells in OVA group was significantly lower in OVA group than in normal control group（P＜0.01）. Levels of IL-10 and TGF-β1 were lower in OVA group than in normal control group（P＜0.05）（P＜0.01）. Conclusions：There are differences in quantities and functions of Treg cells between food allergy and normal mice.