白细胞介素-10基因修饰对骨髓间充质干细胞体外免疫效应的影响

Effects of interleukin-10 modification on in vitro immunosuppression of bone marrow mesenchymal stem cells

摘要:

目的：研究白细胞介素-10（interleukin-10，IL-10）基因修饰的骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMMSCs）体外免疫效应。方法：RT-PCR克隆大鼠IL-10基因并构建逆转录病毒pLNCX-IL-10，转染大鼠BMMSCs；流式细胞仪检测IL-10基因修饰的BMMSCs（BMMSCsIL-10）体外对T淋巴细胞亚群的影响，CCK-8法检测BMMSCsIL-10及其培养上清液对体外混合淋巴细胞增殖的影响。结果：BMMSCsIL-10能明显减少CD4+ T，降低CD4+/CD8+比值，比BMMSCs作用明显（P=0.000）；BMMSCsIL-10及其培养上清液比BMMSCs更能明显抑制T淋巴细胞增殖反应（P=0.000）。结论：IL-10基因修饰能增强BMMSCs的体外免疫抑制作用。

Abstract:

Objective：To study the in vitro immunosuppression of interleukin-10（IL-10） modified bone marrow mesenchymal stem cells（BMMSCsIL-10）. Methods：IL-10 was cloned by RT-PCR and was used to construct pLNCX-IL-10. The constructed retrovirus was transfected into rat BMMSCs. T lymphocyte subsets in vitro influenced by BMMSCsIL-10 were detected by flow cytometry. Effects of BMMSCsIL-10 and its culture supernatant on in vitro mixed heterologous T lymphocyte proliferation were detected by CCK-8. Results：BMMSCsIL-10 significantly reduced CD4+ T and lowered the ratio of CD4+/CD8+，more intense than BMMSCs（P=0.000）. BMMSCsIL-10 and its culture supernatant significantly inhibited T lymphocyte proliferation，more intense than BMMSCs（P=0.000）. Conclusions：IL-10 modification can enhance the in vitro immunosuppressive effect of BMMSCs.