豚鼠过敏性休克死亡白细胞介素-4、白细胞介素-13与免疫球蛋白E的表达及法医学意义

Expression and forensic significance of interleukin-4，interleukin-13 and immunoglobulin E in guinea pigs died of anaphylactic shock

摘要:

目的：建立豚鼠过敏性休克死亡动物模型，探讨豚鼠血清中白细胞介素（interleukin，IL）-4、IL-13和免疫球蛋白E（Im－munoglobulin E，IgE）的表达，旨在为临床和法医学诊断过敏性休克提供科学、客观的参考依据。方法：用酶联免疫吸附测定（en－zyme-linked immunosorbent assay，ELISA）法检测30只过敏性休克豚鼠（实验组20只，对照组10只）血清IL-4、IL-13和IgE的表达情况。结果：实验组血清IL-4水平明显高于对照组（P=0.000）；实验组血清IL-13水平明显高于对照组（P=0.000）；实验组血清IgE水平明显高于对照组（P=0.000）。结论：实验组血清IL-4、IL-13与IgE水平明显高于对照组，为过敏性休克临床诊断及法医学鉴定提供了参考依据。

Abstract:

Objective：To establish animal model of guinea pig anaphylactic shock，to explore the expression of interleukin-4（IL-4），interleukin-13（IL-13） and immunoglobulin E（IgE） in guinea pig serum and to provide scientific and objective evidences for clinical and medicolegal diagnosis of anaphylactic shock as well. Methods：Totally 30 guinea pigs were died of anaphylactic shock were divided into experiment group（n=20） and control group（n=10）. ELISA method was used to detect IL-4、IL-13 and IgE by extracting serum of guinea pigs in experiment group and control group. Results：Serum IL-4 level in experimental group was higher than that in control group，P=0.000；serum IL-13 level in experimental group was higher than that in control group，P=0.000；serum IgE level in experi－mental group was higher than that in control group，P=0.000. Conclusions：Serum levels of IL-4，IL-13 and IgE in experiment group are obviously higher those in control group，which provides evidences for anaphylactic shock forensic identification and clinical diag－nosis.