P300/CBP相关因子在肝细胞癌中的表达及临床意义

Expression of P300/CBP-associated factor in hepatocellular carcinoma and its clinicopathological significance

摘要:

目的：检测P300/CBP相关因子（P300/CBP-associated factor，PCAF）在肝细胞癌（hepatocellular carcinoma，HCC）中的表达情况，分析其与肝癌临床病理特征之间的相关性。方法：收集40例HCC及对应癌旁组织，运用RT-PCR、免疫组化技术检测PCAF在肝癌及对应癌旁组织中的表达情况，研究PCAF蛋白表达与肝癌临床病理特征之间的相关性；应用Western blot技术检测人正常肝细胞LO2和肝癌细胞HepG2和SMMC-7721中PCAF蛋白的表达。结果：PCAF mRNA及蛋白在肝癌组织中的表达低于对应的癌旁组织（P=0.000）；PCAF蛋白低表达与肿瘤肝内转移（rs=0.413，P=0.037）、门脉侵犯（rs=0.589，P=0.011）和高TNM分期（rs=0.513，P=0.029）相关；人肝癌细胞株HepG2和SMMC-7721中PCAF 蛋白的表达较正常肝细胞株LO2降低（P=0.000）。结论：肝癌细胞系及肝癌组织中PCAF表达下调，且PCAF的表达下调与肝癌的恶性病理特征相关。

Abstract:

Objective：To investigate the expression of P300/CBP-associated factor（PCAF） and its correlation with the clinicopatholog－ical features in hepatocellular carcinoma（HCC）. Methods：Forty samples of surgically resected HCC and adjacent noncancerous tis－sues were collected. PCAF expression in the samples was detected using RT-PCR and immunohistochemical staining. Correlation be－tween PCAF protein expression and clinicopathological feature was analyzed. PCAF protein expression in normal hepatic cell line （LO2） and HCC cell lines（HepG2 and SMMC-7721） was detected. Results：mRNA and protein expression of PCAF in HCC tissues was significantly down-regulated compared with that of adjacent noncancerous tissues（P<0.05，respectively）. Clinicopathological analysis suggested that PCAF protein low-expression was associated with tumor intrahepatic metastasis（rs=0.413，P=0.037），portal vein invasion（rs=0.589，P=0.011） and advanced TNM stage（rs=0.513，P=0.029）. Expression of PCAF protein in HCC cell lines HepG2 and SMMC-7721 was significantly lower compared with that in hepatic cell line LO2（P<0.05，respectively）. Conclusions：Expression of PCAF is down-regulated in HCC cell lines and HCC tissues and the low-expression of PCAF is correlated with poor clinico-pathological characteristics of HCC.