阴道加德纳菌通过一种毒素介导输卵管组织损伤

Gardnerella vaginalis mediating fallopian tube tissue injury through a toxin

摘要:

目的：探讨阴道加德纳菌（gardnerella vaginalis，G.vaginalis）及其培养上清对输卵管组织的损伤作用。方法：体外培养不同株的G.vaginalis，制备不同浓度的细菌悬液，对菌液离心后，将上清进行过滤获得无菌的细菌培养上清（细菌滤液），用反复冻融和灭活处理细菌滤液，对牛输卵管组织进行组织培养，用不同浓度梯度的细菌悬液和处理的细菌滤液对牛输卵管组织进行刺激，利用超高倍显微镜观察不同组输卵管组织表面纤毛不同时间点的运动强度与幅度。采用CD59功能性抗体和有丝分裂原活化蛋白激酶p38（mitogen-activated protein kinase p38，p38 MAPK）途径抑制剂判断其对损伤的作用。结果：菌液，无菌细菌滤液，热处理、反复冻融处理的细菌滤液均能对输卵管组织纤毛的运动能力造成损伤，使其运动强度与幅度减弱。经与对照培养基比较差异有统计学意义（P<0.007），组织切片未能发现明显损伤，扫描电镜检查发现输卵管组织黏膜层纤毛组织数量与密度明显下降。CD59功能性抗体和p38 MAPK途径抑制剂能够减弱此种毒素产生的损伤。结论：G.vaginalis通过一种可溶性的毒素经CD59依赖的p38 MAPK途径影响输卵管组织的功能。

Abstract:

Objective：To explore the effect of gardnerella vaginalis（G.vaginalis） and its culture supernatant on the injury of fallopian tube tissue. Methods：Two strains of G.vaginalis were cultured in vitro. Different concentrations of bacterial suspension and sterilie bac－terial filtrate were prepared. Bacterial filtrate was repeatedly frozen thawed and inactivated. Bovine oviduct was cultured with or with－out different concentrations of bacterial suspension and processed bacterial filtrate. Ultra-high-power microscope was used to observe the cilia beating intensity and amplitude at different time points in different groups. Effects of G.vaginalis and different treatment forms of its culture supernatant on fallopian tube were determined. CD59 function antibodies and mitogen-activated protein kinase p38（p38 MAPK） pathway inhibitor was used to determine the damage process. Results：Sterile bacterial filtrate，heat treated bacterial filtrate，repeatedly frozen thawed bacterial filtrate all can cause damage to athletic ability of oviduct cilia and weaken their beating intensity and amplitude（P<0.007）. Tissue sections failed to find evidence of damage，but scanning electron microscope examination found that the number and length of the oviduct mucosal layer cilia were decreased. CD59 functional antibody and p38 MAPK path－way inhibitor can alleviate such toxins damage. Conclusions：G.vaginalis affect the function of the oviduct by a soluble toxin via CD59 dependent p38 MAPK pathway.