成年小鼠实验性心肌梗死后胚胎基因Tbx18的表达情况

Expressions of embryo gene Tbx18 in adult mice after experimental myocardial infarction

摘要:

目的：检测实验性心肌梗死后成年小鼠胚胎基因Tbx18的表达情况，了解成年小鼠心肌损伤后胚胎基因Tbx18的时空表达特点。方法：90只C57BL/6小鼠随机分为假手术组（sham组）及心梗组，用右侧卧位结扎冠状动脉左前降支的方法建立成年小鼠心肌梗死模型，分别用荧光定量PCR和Western blot检测小鼠心肌梗死后1、3、5、7 d心脏梗死和周边区Tbx18 mRNA及蛋白表达情况，并与假手术组相比较。结果：手术组梗死及周边区心肌组织Tbx18 mRNA在心梗后3 d及5 d较假手术组有明显升高（F值分别为2 778.88、462.36，P值均=0.000）；心梗后小鼠心肌组织Tbx18蛋白表达水平在心梗后3 d及5 d明显升高（与心梗后1 d相比，t值分别为7.982、9.307，P值均=0.000），同PCR结果相符；心梗后3 d及5 d免疫荧光结果提示表达部位主要位于梗死区及其周边区域。结论：成年小鼠心肌梗死后胚胎基因Tbx18有一过性激活，心梗后3 d及5 d表达明显，其表达部位主要在梗死及周边区域。

Abstract:

Objective：To examine expressions of embryo gene Tbx18 in adult mice after experimental myocardial infarction（MI）and to study the spatiotemporal characteristics of embryo gene Tbx18 from adult mice with injured myocardium. Methods：Totally 90 C57BL/6 mice were divided into sham group and MI group randomly. MI model of adult mice was induced by ligating the left anterior de－scending coronary artery on right lateral position. Real-time fluorescence quantitative PCR and Western blot were respectively used to measure the mRNA and protein expressions of Tbx18 in infraction and adjacent area of MI group on 1，3，5，7 d after operation，and comparison was made between MI group and sham group. Results：PCR results indicated a remarkable increase of Tbx18 mRNA in infraction and its adjacent tissues of MI group on 3，5 d after operation compared with those in sham group（F=2 778.88，P=0.000；F=462.36，P=0.000）. Results of Western blot showed that expressions of Tbx18 protein in mice infraction tissues reached the peak on 3，5 d after operation（compared those on 1 d after operation，t=7.982，P=0.000；t=9.307，P=0.000），which was consistent with PCR re－sults. Immunofluorescence results on 3，5 d after operation revealed that the expression area mainly located at infraction and its adja－cent area. Conclusions：A transient activation of embryo gene Tbx18 exists at infraction and its peripheral area in the adult mice with MI，especially on 3，5 d after operation.