涂有rhBMP-2的克氏针对长管状骨骨折愈合作用的影响

Effects of rhBMP-2-coated Kirschner wire on the recovery of long tubal bone fracture

摘要:

目的：观察涂有重组人骨形态发生蛋白-2（recombinant human bone morphogenetic protein 2，rhBMP-2）克氏针对骨折愈合影响。方法：36只新西南大白兔，随机分成两组，在其双侧胫骨制作骨折模型，扩髓，实验组胫骨骨折髓腔内插入rhBMP-2/左消旋聚乳酸（poly-D，L-lactide，PDLLA）涂层的克氏针固定骨折，对照组髓腔内插入单纯PDLLA涂层的克氏针固定骨折，术后分别于4、8、12周处死大白兔，取胫骨骨折标本进行骨形态学分析和生物力学测定，比较两组骨折处新骨形成、生物力学情况。结果：实验组新骨形成优于对照组；实验组断裂应力、最大应力高于对照组（P<0.05）。结论：rhBMP-2、PDLLA混合后涂抹在克氏针表面来固定骨折，随着PDLLA降解，缓慢释放rhBMP-2，促进骨折愈合，缩短骨折愈合时间。

Abstract:

Objective：To observe the effects of rhBMP-2-coated Kirschner wire on the recovery of long tubal bone fracture. Methods：Totally 36 new southwest big white rabbits were randomly divided into two groups. Kirschner wire coated with rhBMP-2/poly-D，L-lactide（PDLLA） was inserted into the medullary cavity of fractured tibia in experimental group while Kirschner wire coated with only PDLLA was inserted into the medullary cavity in control group. Bone morphology and bending strength were analyzed and measured at 4，8，12 weeks postoperatively. Results：Osteogenesis was better in experimental group compared with that in control group；fracture stress and maximum stress were higher in experimental group compared with those in control group（P<0.05）. Conclusions：Kirschn－er wire coated with rhBMP-2 and PDLLA can promote the bone fracture recovery and shorten the fracture recovery time with the dis－solution of PDLLA and release of rhBMP-2.