Yes相关蛋白在前列腺癌中的表达及意义

Expression and significance of Yes-associated protein in prostate cancer

摘要:

目的：探讨Yes相关蛋白（Yes-associated protein，YAP）在前列腺癌（prostate cancer，PCa）组织、癌旁（pericarcinoma，PC）组织和良性前列腺增生（benign prostatic hyperplasia，BPH）组织中的表达差异及其意义。方法：采用免疫组化技术检测32例PCa，15例PC和15例BPH中YAP的表达情况；采用Western blot技术检测15例PCa和相应PC中YAP的表达水平。结果：32例PCa中YAP阳性表达25例，阳性率为78.13％；15例PC中YAP阳性表达4例，阳性率为26.67％；15例BPH中未发现YAP阳性表达。组间差异有统计学意义（P=0.000）；YAP的表达与PCa的临床分期、Gleason评分有关（分别为P=0.019；P=0.012）。临床分期越高，Gleason评分越高，YAP的阳性率越高。Western blot显示15例PCa中YAP表达（0.723±0.084）均高于相应PC（0.455±0.161），差异具有统计学意义（P=0.006）。结论：YAP在PCa中的表达明显高于非肿瘤组织，表现出明显的肿瘤特异性，有望成为PCa临床诊断和治疗的一个新指标。

Abstract:

Objective：To investigate the expression and clinical significance of Yes-associated protein（YAP） in tissues of prostate can－cer（PCa），pericarcinoma（PC） and benign prostate hyperplasia（BPH）. Methods：Expression of YAP protein of 32 cases of PCa，15 cases of PC and 15 cases of BPH was investigated by immunohistochemistry. Moreover，different expression of YAP protein of 15 cases of PCa and its corresponding PC tissues were confirmed by Western blot. Results：Rate of YAP positive staining（78.13%，25/32） in PCa tissues was higher than that in PC tissues（26.67%，4/15） and YAP positive staining was not seen in BPH tissues. Staining differences between each group were statistically significant（P=0.000）. Furthermore，different expression of YAP protein was relevant with clinical stage and degree of differentiation（P=0.019，P=0.012）. The higher the clinical stage，the higher the Gleason score and YAP positive staining rate. Western blot demonstrated that YAP protein expression was higher in 15 cases of PCa（0.723±0.084） than in corresponding PC tissues（0.455±0.161），with statistically significant differences（P=0.006）. Conclusions：Expression of YAP protein is significantly higher in PCa tissues than in non-tumorous tissues，demonstrating obvious tumor specificity in PCa. YAP may be a new target in the diagnosis and treatment of PCa.