VE-cadherin在不同侵袭性能非小细胞肺癌细胞株的表达及沉默VE-cadherin对非小细胞肺癌细胞株侵袭、迁移的影响

Expression of VE-cadherin in different non-small cell lung cancer cell lines and effects of VE-cadherin silence on the invasion and metastasis of non-small cell lung cancer cell lines

摘要:

目的：探讨VE-cadherin在不同侵袭性能非小细胞肺癌（non-small cell lung cancer，NSCLC）细胞株的表达及沉默VE-cadherin对NSCLC细胞株侵袭、迁移的影响。方法：通过定量PCR及Western blot检测VE-cadherin在高侵袭性能的NSCLC细胞株PG、中度侵袭性能的NSCLC细胞株H1299及低侵袭性能的NSCLC细胞株PA 中mRNA及蛋白的表达；并通过RNA干扰沉默VE-cadherin的表达来探讨其对NSCLC细胞株PG侵袭、迁移能力的影响。结果：VE-cadherin在3种NSCLC细胞株中都有表达，且其mRNA及蛋白在3种细胞株中的表达强度依次为PG、H1299、PA，PG与H1299相比较，差异有统计学意义（P＝0.00０），PA与PG、H1299相比较，差异均具有统计学意义（P＝0.000）；Transwell小室基质侵袭实验结果显示，干扰组与对照组的侵袭细胞数分别为（34．33±3．06）个和（86．00±7.93）个，组间比较差异有统计学意义（P=0.000）；划痕损伤实验结果显示，对照组与干扰转染组的细胞迁移距离相比，24 h内没有明显差异（P=0.825），而48、72 h内对照组明显高于干扰转染组，差异具有统计学意义（P=0.000）。结论：VE-cadherin可能参与NSCLC的侵袭和迁移，并在此过程中起重要作用；VE-cadherin可能会成为治疗NSCLC侵袭、血管生成和转移的又一新靶点。

Abstract:

Objective：To detect the expression of VE-cadherin in different non-small cell lung cancer cell（NSCLC） lines with distinct invasion performance and to investigate the effects of the VE-cadherin silence on the invasion and metastasis of NSCLC cell lines. Methods：Expression of the VE-cadherin mRNA and protein were detected by quantitative PCR and Western blot in NSCLC cell lines with highly invasive，normally and low invasive ability（PG，H1299 and PA）. Effects of expression of VE-cadherin on the invasion and metastasis of PG cell line were explored by RNA interference. Results：VE-cadherin expressed in 3 kinds of NSCLC cell lines.mRNA and protein expression intensity of VE-cadherin was higher in PG，followed by H1299 and PA cell lines with statistical differences between PG and H1299（P=0.000） and statistical differences between PA and H1299，PG（P=0.000）. Result of Transwell chamber matrigal invasion assays revealed that the numbers of invasive cells of control group and interference group were 34.33 ±3.06 and 86.00±7.93 respectively，with statistical differences between groups（P=0.000）. Results of scratch damage experiment showed that the migration abilities between control group and interference group had no obvious differences in the first 24 h（P=0.825）. But after 48 h or 72 h，metastasis distance of control group was farther than that of interference group with statistical differences（P=0.000）. Invasion and metastasis of PG cell were down regulated by the reduction of the expression of VE-cadherin. Conclusions：VE-cadherin may play a critical role in the invasion and metastasis of NSCLC and may become a new target in the prevention and treatment of invasion，angiogenesis and metastasis of NSCLC.