MGMT和MMP-7在星形细胞瘤中表达及临床意义

Expression and the clinical significance of MGMT and MMP-7 in human astrocytoma

摘要:

目的：研究星形细胞瘤组织中甲基鸟嘌呤DNA甲基转移酶（methylguanine-DNA methyltransferase，MGMT）和基质金属蛋白酶-7（matrix metalloproteinase-7，MMP-7）基因蛋白的表达和意义。方法：应用免疫组化SP法检测49例星形细胞瘤中MGMT和MMP-7基因蛋白的表达。结果：MGMT、MMP-7蛋白在星形细胞瘤中的表达率分别为55.10%（27/49）和51.02%（25/49），MGMT和MMP-7的表达与星形细胞瘤组织病理分级有关（P<0.05），与其他临床因素无关（P >0.05）；MGMT和MMP-7蛋白在星形细胞瘤中的表达率复发组与无复发组有明显差异（P<0.05），MMP-7抗原与MGMT表达呈负相关（rs=-0.488，P<0.01）。结论：MGMT和MMP-7可能共同参与了星形细胞瘤的发生与发展，MGMT的低表达甚至缺失和MMP-7高表达对星形细胞瘤的恶性程度及预后有指导意义。

Abstract:

Objective：To investigate the expression and significance of methylguanine-DNA methyltransferase（MGMT）and matrix metalloproteinase-7（MMP-7） protein in human astrocytoma. Methods：Protein expression of MGMT and MMP-7 was detected in 49 cases of human astrocytoma with SP immunohistochemical method. Results：Expression rate of MGMT was 55.10%（27/49） and those of MMP-7 was 51.02 %（25/49）. Expression of MGMT and MMP-7 protein was related with histological grade（P<0.05），but not re－lated with other clinical factors（P >0.05）. There was significant differences in the MGMT and MMP-7 expression rate between group with recurrence and group without recurrence（P<0.05）. There were significant negative correlations between MGMT and MMP-7 protein expression（rs=-0.488，P<0.01）. Conclusions：MGMT and MMP-7 play important roles in occurrence and development of human astrocytoma. Low expression of MGMT and over expression of MMP-7 provide important implications of malignancy and prognosis for human astrocytoma.