转录因子激活蛋白-2在Buerger’s病中的表达与意义

Expression and significance of transcription factor activator protein-2 in Buerger’s disease

摘要:

目的：观察转录因子激活蛋白-2（transcription factor activator protein-2，AP-2）在Buerger’s病中的表达与分布。方法：临床收集2011年1月至2012年3月共23例行腰交感神经节切除术Buerger’s病患者腰交感神经节标本，应用RT-PCR、Western blot方法检测AP-2在Buerger’s病组中基因和蛋白的表达变化，使用免疫组化的方法检测AP-2在 Buerger’s病组中的表达分布规律，以观察在该组中的表达特点。临床收集12例正常病例标本以同样方法处理。结果：RT-PCR和Western blot结果显示，与正常对照组中相比，Buerger’s病组中AP-2基因和蛋白表达水平具有统计学差异（P<0.05），免疫组化显示病理组AP-2表达高于正常组，主要分布在细胞核。结论：Buerger’s病组中AP-2水平升高，表明AP-2在Buerger’s病的发生与发展过程中可能发挥了重要作用。

Abstract:

Objective：To investigate the expression and distribution of transcription factor activator protein-2（AP-2） in Buerger’s dis－ease. Methods：Lumbar sympathetic ganglia were sampled in a total 23 patients with Buerger’s disease between January 2011 and March 2012. Gene and protein expression of AP-2 in the lumbar sympathetic ganglia neurons was observed by RT-PCR and Western blot. Distribution principles of AP-2 in the lumbar sympathetic ganglia neurons were observed by immunohistochemical methods. Twelve normal lumbar sympathetic ganglia were processed by the same way. Results：RT-PCR and Western blot results demonstrated that AP-2 gene and protein expression levels in Buerger’s disease tissues were significantly different from those in normal tissues（P<0.05）. Immunohistochemical result demonstrated that AP-2 expressions in Buerger’s disease tissues were higher than those in normal tissues，mainly distributed in the nucleus. Conclusions：AP-2 level increases in patients with Buerger’s disease，indicating that AP-2 plays an important role in the occurrence and development of Buerger’s disease.