姜黄素对APPswe/PSEN1dE9双转基因小鼠海马神经元SR-BⅠ和ABCA1表达的影响

Effects of curcumin on the expression of SR-BⅠ and ABCA1 in neurons of hippocampus of APPswe/PSEN1dE9 double transgenic mice

摘要:

目的：观察姜黄素对β-淀粉样前体蛋白swe基因/早老蛋白1dE9基因（APPswe/PSEN1dE9）双转基因小鼠海马神经元B类Ⅰ型清道夫受体（scavenger receptor class B typeⅠ， SR-BⅠ）和三磷酸腺苷结合盒A1（ATP-binding cassette transporter，AB－CA1）表达的影响，探讨姜黄素在阿尔茨海默病（Alzheimer’s disease，AD）防治中的机制。方法：将10只6月龄的APPswe/PSEN1dE9双转基因小鼠随机分为对照组和姜黄素饲喂组，每组5只。姜黄素饲喂组每天饲喂500 ppm姜黄素，6个月后采用免疫组化法检测小鼠海马神经元SR-BⅠ和ABCA1表达的变化。结果：与对照组相比，姜黄素饲喂组小鼠海马神经元ABCA1表达明显增加（t=-10.805，P=0.000），但2组小鼠海马神经元中均未见SR-BⅠ表达。结论：姜黄素能提高APPswe/PSEN1dE9双转基因小鼠海马神经元ABCA1的表达，而SR-BⅠ在神经元中未见表达。

Abstract:

Objective：To observe the effect of curcumin on expression of scavenger receptor class B typeⅠ（SR-BⅠ） and ATP-bind－ing cassette transporter 1（ABCA1） in neurons of hippocampus of APPswe/PSEN1dE9 double transgenic mice and to investigate the mechanism of curcumin in the prevention and treatment of Alzheimer’s disease. Methods：Ten six-month-old APPswe/PSEN1dE9 double transgenic mice were randomly divided into control group and curcumin group． Each group had 5 mice. Mice in curcumin group were fed curcumin 500 ppm every day. Six months later，changes of the expression of SR-BⅠ and ABCA1 in neurons of hippicampus were detected by immunohistochemistry. Results：Expression of ABCA1 in neurons of hippocampus of mice in curcumin group was increased significantly compared with that of control group（t=-10.805，P=0.000） while expression of SR-BⅠ was not detected in both groups. Conclusions：Curcumin can induce expression of ABCA1 in neurons of hippocampus of APPswe/PSEN1dE9 double transgenic mice while expressions of SR-BⅠ can not be detected in neurons.